New Technologies for Glutamate Interaction

Visualization of Glutamatergic Neurotransmission in Diverse Model Organisms with Genetically Encoded Indicators.- Surface Glutamate Receptors Nanoscale Organization with Super-Resolution Microscopy (dSTORM).- Ligand-Directed Chemical Labeling for Visualizing and Analyzing AMPA Receptors in Neurons.- Live FRET-FLIM Imaging to Study Metabotropic Signaling via the NMDA Receptor.- Quantitative Analysis of Single Glutamatergic Vesicles in the Brain.- Imaging Microtubule Network in Rodent Giant Glutamatergic Presynaptic Terminals.- Photooxidation of Genetically Encoded MiniSOG-Fused VGLUT2 for Identification of Glutamatergic Synapses by Transmission and 3D Electron Microscopy.- Chemogenetic Approaches to Study Astrocytes at Glutamatergic Synapses.- Studying the Role of Astrocytes at Synapses using Single-Cell Transcriptomics.- Analysis of Synaptic Glutamate Clearance as a Possible Indicator of Synaptic Health in the Degenerating Rodent Brain.- Computational Models of Astrocyte Function at Glutamatergic Synapses.- Electrophysiological Recordings of Oligodendroglia in Adult Mouse Brain Slices.- Synaptic Integration at Neuron-OPC Synapses.- Mapping Synaptic Inputs to Oligodendroglial Cells using In Vivo Monosynaptic Viral Tracing.- In Vivo Viral Gene Delivery to Manipulate Functional Properties of AMPA Receptors in Oligodendrocyte Lineage Cells.- Studying Synaptic Integration of Glioma Cells into Neural Circuits.
  

This volume explores the latest technologies used to study the co-existence of neuronal and neuron-glia synapses in the brain. The chapters in this volume are organized into three parts. Part One covers recent advancements in the technical approaches for studying glutamatergic signalling between neurons. Part Two highlights approaches to study the functional role of astrocytes at neuronal synapses. Part Three focuses on fast signalling at neuron-glia synapses, and highlights relevant methods for investigating these unusual synapses, including slice electrophysiology and in vivo gene delivery techniques. In the Neuromethods series style, all chapters contain key advices from experts in the field that are valuable for performing successful experiments on the topic in your laboratory.

Cutting-edge and thorough, New Technologies for Glutamate Interactions: Neurons and Glia is a valuable resource for both neuronal and glial physiologists who are interested in learning about and developing new methods to further understand neuronal and neuron-glia synaptic transmission.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts