Neural Crest Cells
Editat de Hisayoshi Yoshizakien Limba Engleză Hardback – 20 noi 2026
This volume provides a practical, comprehensive guide to studying neural crest cells in a variety of model organisms and research modalities. Chapters guide readers through basic concepts and core protocols, in vivo imaging and model organisms, molecular, cellular, bioinformatics analyses, and disease models and translational applications. Written for the highly successfulMethods in Molecular Biologyseries, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and practical,Neural Crest Cells aims to be a versatile resource for exploring every facet of neural crest cell research-from the basics of isolating and labeling these cells to high-end imaging techniques, functional assays, and the latest omics-based analyses.
Chapter 5 is available open access under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License via link.springer.com.
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Specificații
Cuprins
Molecular Mechanisms of Neural Crest Development and Methodological Interventions.- In ovo gene transfer into avian neural crest cells by electroporation.- Gene transfer into neural crest cells in mouse embryos.- RNA Whole-Mount In Situ Hybridization for Mouse Embryos.- Whole-mount fluorescent immunostaining of mouse embryos.- Ex ovo bead implantation into the neural tube to modulate the migration of neural crest cells in chicken embryos.- Imaging Embryonic Anatomy: Skeletal Staining and Three-Dimensional Reconstruction from Serial Histological Sections.- Generating neural crest fluorescent reporter line in Zebrafish.- Experimental and Computational Procedures for Single-cell Gene Expression Analysis of Cardiopharyngeal Neural Crest Cells.- Experimental and Computational Protocols for Single-Nucleus Multiome (ATAC + Gene Expression) Analysis of Cardiopharyngeal Neural Crest Cells.- Single-Cell Analysis in Python: A Practical Pipeline from Data Preprocessing to Clustering.- Voxel-wise Quantitative Analysis of 3D Immunofluorescence Images in.- Neurocristopathies: From Molecular Pathogenesis to Regenerative Medicine.- In Vivo Modeling of Neurocristopathy-Associated Mutation Using CRISPR/Cas9 Genome Editing.- Efficient Isolation and Neurosphere Culture of Mouse Enteric Neural Crest Cells (ENCCs).- A Method for the Transplantation of Enteric Neural Stem Cells into the Murine Gut.- Optogenetic stimulation and recording of transplanted ENCC activity using an ex vivo organ bath.- In Ovo Chicken Chorioallantoic Membrane Assays Using Neuroblastoma.- A tumorigenesis model of osteosarcoma and neuroblastoma derived from human iPSC-derived cranial neural crest cells.- Induction of Neural Crest Cells from Human iPSCs.- Generation of Mesenchymal Stem/Stromal Cells from Human iPSCs via Neural Crest Cells.- Generation of Jawbone-like Organoids from Human iPS cells through Neural Crest Cells.