This fully updated and revised second edition covers nanofluidics with a focus on bioanalytical applications. Each chapter now features methodology and "tips and tricks" sections and two new chapters examine new applications of nanotechnology. The prominent editors draw on an international authorship and with 20% more illustrations than the first edition, the book, written at a level accessible to experts and non-experts alike, is essential reading for all advanced nanobiotechnology courses in academic institutions.