Muscle Stem Cells
Editat de Nathalie Didier, Caroline E Brunen Limba Engleză Hardback – 3 noi 2026
This detailed volume provides an experimental roadmap for interrogating muscle stem cell biology, from tissue dissociation and cell isolation to ex vivo analysis, in vitro modeling, functional assays, and multi-omics technologies. The book explores how to capture or preserve the physiological state of muscle stem cells in situ and ex vivo, techniques for in situ imaging, computational analysis of immunofluorescence, cross-grafting, and co-culture with niche-associated cells, as well as protocols applicable to human muscle stem cells and skeletal muscle tissue and approaches to generate human muscle progenitors and model human muscle stem cell fate in a dish. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and practical, Muscle Stem Cells: Methods and Protocols serves as a useful resource for both new and experienced investigators working in the field of muscle stem cell biology in order to facilitate rigorous experimentation, methodological innovation, and further discovery in skeletal muscle regeneration.
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Specificații
Cuprins
Optimized Dissociation of Skeletal Muscle Tissue to Recover Muscle Stem Cells.- Isolation of Mouse, Rat, and Human Muscle Stem Cells by Fluorescence Activated Cell Sorting Based on a7-integrin.- Co-Cultures of Macrophages with Muscle Stem Cells or Fibroadipogenic Precursor Cells from Skeletal Muscle.- Tissue Clearing and Immunostaining of Mouse and Human Skeletal Muscles for In Situ Analysis of Muscle Stem Cell Spatial Organization.- Automated Bioinformatic Analysis of Skeletal Muscle Immunofluorescence for the Quantification of Muscle Stem Cells.- Spatial and Quantitative Immunofluorescence Analysis of Human Myogenic Cells in Mouse Skeletal Muscle Xenografts.- Ex Vivo Analysis of Muscle Stem Cells on Single EDL Myofibers, from Quiescence to Self-Renewal.- An Ex Vivo Cross-Grafting and Live-Imaging Assay to Analyze Myofiber Niche Impact on Muscle Stem Cells.- Efficient In Vitro Generation and Isolation of Quiescent Pax7¿ Human Muscle Reserve Cells.- Modeling Skeletal Muscle Stem Cell Fate In Vitro.- Automated Quantitative Analysis of Myogenesis in Isolated Mouse and Rat Muscle Stem Cells.- Directed Differentiation of Human Pluripotent Stem Cells into Skeletal Muscle Progenitor Cells.- Flow Cytometry Analysis of Mitochondrial Function in Muscle Stem Cells.- Measurement of Oxygen Consumption Rate of Primary Mouse Skeletal Muscle Stem Cells and Their Progeny on the Seahorse Analyzer.- Low Input RNA Sequencing of Freshly Isolated Muscle Stem Cells.- Low-Input RNA-Sequencing of In Situ Fixed Adult Murine Muscle Stem Cells.- Low-Input Proteomic Analysis of In Situ Fixed Adult Murine Muscle Stem Cells.- Bioinformatic Workflow for the Analysis of Single-Nucleus RNA-Sequencing Data from Skeletal Muscle.- Chromatin Immunoprecipitation Followed by PCR (ChIP-PCR) from Prospectively Isolated Murine Muscle Stem Cells.- Optimized Low-Input ATAC-Seq Protocol for Freshly Sorted Muscle Stem Cells.- Targeted Chromatin Profiling in Muscle Stem Cells Using a CUT&RUN-qPCR Strategy.- High-Throughput Chromosome Conformation Capture Immunoprecipitation in Skeletal Muscle Stem Cells.