Methods in Microbiology Volume 59: Mpox provides a comprehensive and up-to-date overview of the methodologies, surveillance tools, and analytical approaches shaping contemporary mpox research and public health response. Covering topics from wastewater-based detection and community-level monitoring to clade-specific molecular diagnostics and genomic sequencing, the volume highlights practical techniques for studying viral transmission, evolution, and epidemiology in both high- and low-resource settings. It also explores mathematical and stochastic modelling of human and rodent populations, alongside clinically focused chapters detailing gastrointestinal, neurological, dermatological, and reproductive complications of mpox infection. The volume concludes with advances in structural biology and imaging that elucidate viral mechanisms at molecular resolution, making this an essential reference for microbiologists, clinicians, epidemiologists, and researchers working to strengthen global mpox preparedness and response.

• Integrates cutting-edge surveillance methods—including wastewater monitoring, community-based STI surveillance, and clade-level molecular diagnostics—to provide a comprehensive toolkit for detecting and tracking mpox in diverse settings.
• Bridges laboratory science with clinical and epidemiological insights, offering protocols for genomic sequencing, modelling approaches, and detailed examinations of organ-specific complications to support both research and public health practice.
• Highlights advanced structural and imaging techniques that reveal mpox viral architecture and mechanisms, enabling deeper understanding of viral biology and supporting the development of targeted therapeutics and vaccines.