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Microwave Assisted Proteomics

Autor Jennie R. Lill
en Limba Engleză Hardback – 14 mai 2009
This book is an introduction to microwave-assisted bio-analytical methods with an emphasis on sample preparation for proteomic analyses. Its objective is to provide a history and background for the methods described and the book summarizes the development and application of these protocols from both the literature in this field as well as from the experiences of a variety of laboratories conducting microwave-assisted protocols. It describes the evolution and integration of microwave energy into the biosciences with particular emphasis on the proteomic arena and an in depth evaluation of a variety of techniques within the field of proteomics which benefit from microwave-irradiation is given.
In addition to describing the evolution of the microwave-assisted methodologies, the book also offers a concise protocol section with practical advice on how to implement these methods in any laboratory. Mechanisms of action as well as an overview of the types of instrumentation available are described to help the reader develop the best experimental design for their own budget and experimental aims.
Microwave-assisted proteomics is a rapidly evolving field and this book captures the main areas that fall under this emerging arena.
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Specificații

ISBN-13: 9780854041947
ISBN-10: 085404194X
Pagini: 125
Dimensiuni: 163 x 236 x 19 mm
Greutate: 0.38 kg
Ediția:2009
Editura: Royal Society Of Chemistry
Colecția Royal Society of Chemistry
Locul publicării:Cambridge, United States

Public țintă

Professional/practitioner

Descriere

This book is an introduction to microwave-assisted bio-analytical methods with an emphasis on sample preparation for proteomic analyses. Its objective is to provide a history and background for the methods described and the book summarizes the development and application of these protocols from both the literature in this field as well as from the experiences of a variety of laboratories conducting microwave-assisted protocols. It describes the evolution and integration of microwave energy into the biosciences with particular emphasis on the proteomic arena and an in depth evaluation of a variety of techniques within the field of proteomics which benefit from microwave-irradiation is given.

In addition to describing the evolution of the microwave-assisted methodologies, the book also offers a concise protocol section with practical advice on how to implement these methods in any laboratory. Mechanisms of action as well as an overview of the types of instrumentation available are described to help the reader develop the best experimental design for their own budget and experimental aims.

Microwave-assisted proteomics is a rapidly evolving field and this book captures the main areas that fall under this emerging arena.

Cuprins

Evolution of Microwave Irradiation and Its Introduction to the Biosciences.- Microwave Instrumentation in the Biosciences.- Mechanisms of Microwave-Assisted Action.- Microwave-Assisted Enzymatic Digestions.- Microwave-Assisted Chemical Digestion of Proteins.- Microwave-Assisted Acid Hydrolysis.- Microwave-Assisted Discovery and Characterization of Posttranslational Modifications.- Recent Microwave-Assisted Applications in the Life Sciences.- Epilogue: To Microwave or Not To Microwave?

Caracteristici

Provides an overview of the history of microwave-assisted proteomics
Provides an in-depth discussion on several microwave-assisted methods including enzymatic and chemical proteolytic reactions, microwave-assisted acid hydrolysis for N-terminal sequencing and for amino acid analysis
Includes a discussion of the mechanisms involved
An overview of instrumentation types and limitations
A practical protocol section for the researcher to carry out the experiments in their own laboratory

Textul de pe ultima copertă

This book is an introduction to microwave-assisted bio-analytical methods with an emphasis on sample preparation for proteomic analyses. Its objective is to provide a history and background for the methods described and the book summarizes the development and application of these protocols from both the literature in this field as well as from the experiences of a variety of laboratories conducting microwave-assisted protocols. In addition to describing the evolution of the microwave-assisted methodologies, it includes mechanisms of action as well as an overview of the types of instrumentation available to help the reader develop the best experimental design for their own budget and experimental aims. The key features of this book: * An overview of the history of microwave-assisted proteomics * An in-depth discussion on several microwave-assisted methods including - enzymatic and chemical proteolytic reactions, microwave-assisted acid hydrolysis for N-terminal sequencing and for amino acid analysis * A discussion of the mechanisms involved * An overview of instrumentation types and limitations * A concise and practical protocol section for the researcher to carry out the experiments in their own laboratory Microwave-assisted proteomics is a rapidly evolving field and this book captures the main areas that fall under this emerging arena.