This volume provides a wide range of methods to discover and assess lysosome structure and function are provided. Chapters detail the essential methodologies and giving expert insight to key features with notes pertaining to key elements of the methods. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Lysosomes: Methods and Protocols aims to be comprehensive guide for researchers in the field.