This book constitutes the refereed proceedings of the 8th International Conference on Independent Component Analysis and Signal Separation, ICA 2009, held in Paraty, Brazil, in March 2009. The 97 revised papers presented were carefully reviewed and selected from 137 submissions. The papers are organized in topical sections on theory, algorithms and architectures, biomedical applications, image processing, speech and audio processing, other applications, as well as a special session on evaluation.