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Immunoproteomics

Editat de Kelly M. Fulton, Susan M. Twine
en Limba Engleză Hardback – 15 oct 2025
Providing current diverse approaches and techniques used to study the immunoproteome, Immunoproteomics: Methods and Protocols collects chapters from key researchers that deliver information to be used in diagnostics, disease progression, and vaccine correlates of protection analysis, to name but a few. This detailed volume includes techniques used for the study of the antibody targets of bacterial pathogens, viruses, and cancer, mass spectrometry-based approaches to characterize T-cell epitopes, chapters on detection and relative quantification of cytokines in serum, as well as in silico prediction of epitopes using sequence-based or modeling approaches.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Practical and thorough, Immunoproteomics: Methods and Protocols aids researchers in transferring these techniques to their own laboratories in addition to providing a reference to guide researchers toward appropriate techniques.
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Specificații

ISBN-13: 9781071648315
ISBN-10: 1071648314
Pagini: 480
Ilustrații: XIII, 469 p. 96 illus., 82 illus. in color.
Dimensiuni: 183 x 260 x 32 mm
Greutate: 1.04 kg
Ediția:Third Edition 2026
Editura: Springer

Cuprins

Introduction to the Immune System.- Immunoproteomics Methods and Techniques.- Classical Immunoproteomics: Serological Proteome Analysis (SERPA) for Antigen Identification.- Profiling of Cytokine and Chemokine Responses using Multiplex Bead Array Technology.- Preparation of the Low Molecular Weight Serum Proteome for Mass Spectrometry Analysis.- Enriching for Low Abundance Serum Proteins using ProteoMiner™ and Protein Level HPLC.- Identification of the Antigen Content of Electroimmunoprecipitates.- Detection of Human Norovirus-Specific Antibodies using the Luciferase Immunoprecipitation System (LIPS) Assay.- Intact Mass Spectrometry Analysis of Immuno-Isolated Human Therapeutic Antibodies from Serum.- Antigen Identification for Cell-Binding Antibodies using Ligand Directed Crosslinking and Biotin Transfer.- Whole Genome-Phage Display Libraries: A Powerful Tool for Antigen Discovery.- Multiplexed Detection of Autoantibodies to Glycopeptides using Microarray.- Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope Identification.- Isolation of Major Histocompatibility Complex (MHC)-Associated Peptides by Immunoaffinity Purification.- Enhanced Mass Spectrometry Detection of MHC Peptides.- Enrichment of Phosphorylated MHC Peptides with Immobilized Metal Affinity Chromatography and Titanium Dioxide Particles.- Mass Spectrometric Identification and Molecular Modeling of Glycopeptides Presented by MHC Class I and II Processing Pathways.- Genome Based Bioinformatic Prediction of Major Histocompatibility (MHC).- Enhancing Mass Spectrometry-Based MHC-I Peptide Identification through a Targeted Database Search Approach.- Prioritization of Therapeutic Targets of Inflammation Using Proteomics, Bioinformatics, and In Silico Cell-Cell Interactomics.- Genomics Driven Immunoproteomics: An Integrative Platform to Uncover Important Biomarkers for Human Diseases.- Identification of Antigens in Immune Complexes.- Analysis and Characterization of Immune Cells and their Activation Status by Whole-Cell MALDI TOF Mass Spectrometry.- A Molecular Immunoproteomics Approach to Assess the Viral Antigenicity of Influenza.- Immunoproteomic Biomarkers: From Publication to Personalized Medicine.


Textul de pe ultima copertă

Providing current diverse approaches and techniques used to study the immunoproteome, Immunoproteomics: Methods and Protocols collects chapters from key researchers that deliver information to be used in diagnostics, disease progression, and vaccine correlates of protection analysis, to name but a few. This detailed volume includes techniques used for the study of the antibody targets of bacterial pathogens, viruses, and cancer, mass spectrometry-based approaches to characterize T-cell epitopes, chapters on detection and relative quantification of cytokines in serum, as well as in silico prediction of epitopes using sequence-based or modeling approaches.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Practical and thorough, Immunoproteomics: Methods and Protocols aids researchers in transferring these techniques to their own laboratories in addition to providing a reference to guide researchers toward appropriate techniques.

Caracteristici

Provides current diverse approaches and techniques to study the immunoproteome Offers lab-ready protocols proven and used by experts around the world Features key tips and implementation advice to ensure successful results Includes supplementary material: sn.pub/extras