Colon targeted micro capsules of Tinidazole were prepared by using Guar gum as the drug targeting polymer. The emulsion polymerization technique for preparation of the micro capsules was optimized using full factorial DOE. It was identified that the HLB value of the surfactant mixture was critical for maximizing entrapment efficiency. The in vitro drug release studies in pH 1.2, pH 4.5, pH 6.8 and pH 7.2 buffers indicated no significant drug release at any of these media. However, in pH 6.8 and pH 7.2 buffer enriched with Alpha galactosidase and Galactomannase enzymes, the drug release is complete. This indicates that the delivery system is designed to target the drug release in the Colon region where the release shall be triggered by the microorganisms present in the colon and long intestine.