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Gene Expression Profiling de Richard A. Shimkets

Gene Expression Profiling: Methods in Molecular Biology, cartea 258

Editat de Richard A. Shimkets
en Limba Engleză Hardback – 24 feb 2004
Leading scientists in gene expression methodology and bioinformatics data analysis describe readily reproducible methods for measuring RNA levels in cells and tissues. The techniques presented include new methods for applying the Affymetrix GeneChip®, SAR-SAGE, StaRT-PCR, SSH, the Invader Assay®, and ADGEM. The authors also provide critical bioinformatics insight and resources for data analysis and management. By distilling the basic underlying principles of many methods to a few straightforward concepts, investigators can easily choose the method most appropriate to their application.
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Specificații

ISBN-13: 9781588292209
ISBN-10: 1588292207
Pagini: 180
Ilustrații: X, 168 p.
Dimensiuni: 160 x 241 x 16 mm
Greutate: 0.48 kg
Ediția:2004
Editura: Humana Press Inc.
Colecția Methods in Molecular Biology
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Research

Cuprins

Technical Considerations in Quantitating Gene Expression.- Gene Expression Quantitation Technology Summary.- Standardized RT-PCR and the Standardized Expression Measurement Center.- GeneCalling.- Invader Assay for RNA Quantitation.- Monitoring Eukaryotic Gene Expression Using Oligonucleotide Microarrays.- Amplified Differential Gene Expression Microarray.- Suppression Subtractive Hybridization.- Small Amplified RNA-SAGE.- Gene Expression Informatics.

Textul de pe ultima copertă

The transcription of messenger RNA from a DNA template is a key process in a wide variety of biological systems. In Gene Expression Profiling: Methods and Protocols, leaders in gene expression methodology and bioinformatics data analysis share their best methods for measuring RNA levels in cells and tissues. Each proven protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Special care has been taken to distill the basic underlying principles of each method to a few straightforward concepts so that investigators can easily choose the method most appropriate to their application. The readily reproducible techniques presented include new methods for applying the GeneChip®, SAR-SAGE, StaRT-PCR, SSH, the Invader Assay®, and ADGEM. The authors also provide critical bioinformatics insight and resources for data analysis and management.
Cutting-edge and highly practical, Gene Expression Profiling: Methods and Protocols allows novice and experienced investigators alike not only to choose the best method to determine how genes are turned on and off in many different organisms, but also to use informatics resources to analyze and understand the results.