This detailed volume highlights cutting-edge strategies and technologies that enable scientists to engineer proteins with the objective of visualizing and manipulating cells. From imaging intracellular molecules to manipulating cellular behavior with accuracy, this book explores how engineered proteins are redefining biological research, opening new horizons for understanding protein interactions, disease management, sustainability, and innovation, and what is possible for larger societal benefits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and practical, Engineering Proteins to Visualize and Manipulate Cells aims to stimulate new perspectives on cell visualization and manipulation and advance the boundaries of biological discovery.