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DNA Replication: Methods and Protocols de Sonya Vengrova

DNA Replication: Methods and Protocols: Methods in Molecular Biology, cartea 1300

Editat de Sonya Vengrova, Jacob Dalgaard
en Limba Engleză Paperback – 29 oct 2016
Updated and revised, this thorough volume covers a range of methods focusing on systems, including mammalian, yeast, bacterial and archaeal. This second edition of DNA Replication: Methods and Protocols describes approaches to analyze whole genomes to single molecules, as well as both in vivo and in vitro experiments. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, DNA Replication: Methods and Protocols, Second Edition provides a collections of methods intended for newcomers to this research field and for established laboratories.
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Specificații

ISBN-13: 9781493955916
ISBN-10: 1493955918
Pagini: 295
Ilustrații: X, 295 p. 36 illus., 15 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.54 kg
Ediția:Softcover reprint of the original 2nd ed. 2015
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

A High-Throughput Confocal Fluorescence Microscopy Platform to Study DNA Replication Stress in Yeast Cells.- Microscopy Techniques to Examine DNA Replication in Fission Yeast.- High-Resolution Analysis of Mammalian DNA Replication Units.- Analysing the Dynamics of DNA Replication In Mammalian Cells Using DNA Combing.- Measuring DNA Content by Flow Cytometry in Fission Yeast.- Incorporation of Thymidine Analogs for Studying Replication Kinetics In Fission Yeast.- Edu Incorporation for FACS and Microscopy Analysis of DNA Replication in Budding Yeast.- Determination Of Deoxyribonucleoside Triphosphate Concentrations in Yeast Cells by Strong Anion-Exchange High-Performance Liquid Chromatography Coupled with Ultraviolet Detection.- Measuring Ribonucleotide Incorporation Into DNA In Vitro and In Vivo.- Detection And Sequencing Of Okazaki Fragments In S. Cerevisiae.- Chip-Seq To Analyze The Binding Of Replication Proteins To Chromatin.- Chromatin Immunoprecipitation To Detect DNA Replication And Repair Factors.- Molecular Genetic Methods To Study DNA Replication Protein Function In Haloferax Volcanii, A Model Archaeal Organism.- Single-Molecule Observation Of Prokaryotic DNA Replication.- Analysing the Response to Dysfunction Replication Forks Using the RTS1 Barrier System in Fission Yeast.- Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis.- Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes.

Textul de pe ultima copertă

Updated and revised, this thorough volume covers a range of methods focusing on systems, including mammalian, yeast, bacterial and archaeal. This second edition of DNA Replication: Methods and Protocols describes approaches to analyze whole genomes to single molecules, as well as both in vivo and in vitro experiments.  As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and cutting-edge, DNA Replication: Methods and Protocols, Second Edition provides a collections of methods intended for newcomers to this research field and for established laboratories.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts