Design and applications of cell-free systems –Part A represents an important component in the field of biochemistry, duly acknowledging the pioneering effort made by Nobel laureate Eduard Buchner for discovery of fermentation in yeast cell extracts (YCE). CFS requires a core molecular machinery including RNA polymerase, translational apparatus (ribosomes, tRNA synthases, and translation factors), energy-generating molecules, and their cofactors, substrates, and DNA or plasmid templates for obtaining desired products. CFS is a simple, rapid, and sensitive platform, which is devoid of membrane-bound barriers, yet contains all the mandatory substrates, biomolecules, and machineries required for the synthesis of the desired proteins. It has the potential to overcome loopholes in the current in vivo production systems and it is promising tool in both basic and applied scientific research. It facilitates a simplified organization of desired experiments with a variety of reaction conditions, making CFS a powerful tool in biological and translational science research.

This volume offers wide range of topics on different aspects of cell free systems design and their applications. It covers introduction to applications of cell-free systems, protocol for cell free design, transcription and translation, computational biology, artificial intelligence, automation and robotics for cell free systems, cell free for biosensor, low cost diagnostics, cell free based high throughput screening, biodesign and incorporation of non-natural amino acids into proteins. This volume helps students, researchers, scientists, clinicians, stakeholders, policymakers, and practitioners to understand cell-free systems and their applications in many areas.

• Basic concept and design of cell free systems
• Computational tools, AI, automation and robotics
• Cell free systems for biosensor, diagnostics, high throughput screening and NNAC incorporation