Cytokine Protocols: Methods in Molecular Biology, cartea 249
Editat de Marc De Leyen Limba Engleză Paperback – 10 noi 2010
Authoritative and practical, Cytokine Protocols: Second Edition seeks to aid scientists in furthering the crucially important advancement of cytokine research.”
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Specificații
ISBN-13: 9781617372698
ISBN-10: 1617372692
Pagini: 240
Ilustrații: XIII, 240 p.
Dimensiuni: 152 x 229 x 15 mm
Greutate: 0.38 kg
Ediția:Softcover reprint of hardcover 1st ed. 2004
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:Totowa, NJ, United States
ISBN-10: 1617372692
Pagini: 240
Ilustrații: XIII, 240 p.
Dimensiuni: 152 x 229 x 15 mm
Greutate: 0.38 kg
Ediția:Softcover reprint of hardcover 1st ed. 2004
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:Totowa, NJ, United States
Public țintă
ResearchCuprins
Large-Scale Generation of Plasmids that Express Type I Interferon.- Identification of trans-Acting Factors by Electrophoretic Mobility Shift Assay.- Modulation of the Interferon-? Signal by Transfection of Cells with an Antisense-RNA Expressing Vector.- Competitive RT-PCR to Quantify Small Amounts of mRNA.- In SituHybridization for Cytokines in Human Tissue Biopsies.- Purification, Identification, and Synthesis of Chemokines.- Receptor Isolation and Characterization.- Crystallization of Cytokine-Receptor Complexes.- Biosensor Analysis of Receptor-Ligand Interactions.- Analysis of SH2 Ligands and Identification of Sites of Interaction.- Assays for Antiviral Activity.- Assays for Cytotoxicity.- Assays for Chemotaxis.- In Vitro and In Vivo Assays for High-Molecular-Weight Pegylated Muteins of Granulocyte Colony-Stimulating Factor.- Development of a Mammalian Tet-On Expression Cell Line.- Immunohistochemical Detection of Cytokines in Human Tissue Sections.- Detection of Cytokine- and Chemokine-Expressing Cells at the Single Cell Level.- Detection of Cytokine Signal Transduction “Cross-Talk” in Leukocyte Activation.- Assay System for the Effect of Prostaglandin E2 in the Determination of Polarized Cytokine Production.
Textul de pe ultima copertă
Because cytokines regulate many different bodily functions in living organisms, mainly at the level of cell-cell communications, there is great interest in understanding their mode of action. In Cytokine Protocols, established researchers, physicians, and clinicians present their best biochemical, cellular, and molecular techniques for unraveling and quantifying the events occurring between the initial contact of a cytokine at the membrane receptor and the eventual activation of gene transcription. Described in step-by-step detail to ensure successful experimental results, these protocols cover the large-scale generation and purification of plasmid DNA, the identification of DNA-protein interactions via the gel mobility shift assay, RNA-level phenomena, and the isolation and characterization of cytokines, cytokine-related proteins, and their interactions. The techniques used include the generation of transfectants, the immunohistochemical detection of cytokines in tissue sections, and optimized staining for cytoplasmic detection. Highlights include RT-PCR of small amounts of mRNA, in situ hybridization, biosensor analysis, measurement of biological activities and standardization, immunohistochemical and single-cell detection, and receptor isolation, characterization, and crystallization. Each protocol includes a background introduction, equipment and reagent lists, tips on troubleshooting and avoiding pitfalls, and, where needed, a discussion of the interpretation of results.
Comprehensive and highly practical, Cytokine Protocols offers novice researchers a concise introduction to the principles of cytokine research, and experienced investigators all the advanced techniques and time-saving tips needed to ensure productive research.
Comprehensive and highly practical, Cytokine Protocols offers novice researchers a concise introduction to the principles of cytokine research, and experienced investigators all the advanced techniques and time-saving tips needed to ensure productive research.
Caracteristici
Includes supplementary material: sn.pub/extras