Cantitate/Preț
Produs
Total produse
Vezi coșul
Cytochrome P450 de Zhengyin Yan

Cytochrome P450: Methods in Pharmacology and Toxicology

Editat de Zhengyin Yan, Gary W. Caldwell
en Limba Engleză Paperback – 10 iul 2022
This collection explores detailed experimental protocols necessary for setting up a variety of in vitro cytochrome P450 (CYP) assays that are vital in selecting drug candidates in a drug discovery pipeline. Major factors affecting drug metabolism include CYP expression levels, kinetic parameters for individual CYP enzymes, CYP inhibition and induction, time-dependent inhibition (TDI), CYP stability, non-CYP stability, UDP-glucuronosyltransferases (UGT) stability, excretion mechanisms, and drug-drug interactions (DDI), all addressed in this volume. Written for the Methods in Pharmacology and Toxicology series, chapters include helpful background information on the in vitro assay, a list of all the materials, reagents, and equipment necessary to carry out the assay, a step-by-step protocol, notes containing common and unexpected experimental problems in the assay, as well as references containing important supplementary reading. 

Authoritativeand practical, Cytochrome P450: In Vitro Methods and Protocols serves as a key guide for researchers in the area of discovery and development of new medicines. 
Citește tot Restrânge

Din seria Methods in Pharmacology and Toxicology

  • 5% Histamine Receptors as Drug Targets
    Preț: 108606 lei
  • 5% Drug-Induced Liver Toxicity
    Preț: 91424 lei
  • 5% Nanomaterials in Pharmacology
    Preț: 126603 lei
  • 5% Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases
    Preț: 69807 lei
  • 5% G Protein-Coupled Receptor Kinases
    Preț: 70740 lei
  • 5% Cardiac Drug Development Guide
    Preț: 101718 lei
  • 5% Integrin Targeting Systems for Tumor Diagnosis and Therapy
    Preț: 70005 lei
  • 5% Cytokines in Human Health: Immunotoxicology, Pathology, and Therapeutic Applications
    Preț: 106372 lei
  • 5% Evidence-Based Pharmacovigilance
    Preț: 139955 lei
  • 5% Optimization in Drug Discovery
    Preț: 139488 lei
  • 5% In Vitro Toxicology Systems
    Preț: 156880 lei
  • 5% Antiepileptic Drug Discovery: Novel Approaches
    Preț: 70706 lei
  • 5% Ocular Pharmacology and Toxicology
    Preț: 125449 lei
  • 5% TRP Channels in Drug Discovery
    Preț: 132013 lei
  • 5% Developmental and Reproductive Toxicology
    Preț: 107137 lei
  • 5% Computational Systems Toxicology
    Preț: 75903 lei
  • 5% Biomarker Methods in Drug Discovery and Development
    Preț: 109232 lei
  • 5% Multi-Target Drug Design Using Chem-Bioinformatic Approaches
    Preț: 156761 lei
  • 5% Antibiotic Pharmacodynamics
    Preț: 109619 lei
  • 5% Apoptosis Methods in Toxicology
    Preț: 100543 lei
  • 5% Apoptosis Methods in Pharmacology and Toxicology: Approaches to Measurement and Quantification
    Preț: 68108 lei
  • 5% Herbal Medicines
    Preț: 100714 lei
  • 5% Cancer Prevention
    Preț: 106965 lei
  • 5% Stem Cell-Derived Models in Toxicology
    Preț: 70410 lei
  • 5% G Protein-Coupled Receptor Genetics
    Preț: 107899 lei
  • 5% The Handbook of Metabolomics
    Preț: 108323 lei
  • 5% Genotoxicity and DNA Repair
    Preț: 108323 lei
  • 5% Label-Free Biosensor Methods in Drug Discovery
    Preț: 71164 lei
  • 5% Toxicity and Biodegradation Testing
    Preț: 107163 lei
  • 5% In Vitro Neurotoxicology: Principles and Challenges
    Preț: 69937 lei
  • 5% Process Simulation and Data Modeling in Solid Oral Drug Development and Manufacture
    Preț: 75315 lei
  • 5% Ion Channel Localization
    Preț: 107358 lei
  • 5% Methods for Stability Testing of Pharmaceuticals
    Preț: 83107 lei
  • 5% Computer-Aided Drug Discovery
    Preț: 100992 lei

Preț: 82481 lei

Preț vechi: 86822 lei
-5%

Puncte Express: 1237
Paperback 82481 lei
Hardback 125842 lei

Carte tipărită la comandă

Livrare economică 05-19 iunie

 Adaugă în coș

Wish list
Gift list
Am citit!
Adaugă în listă

Specificații

ISBN-13: 9781071615447
ISBN-10: 1071615440
Pagini: 392
Ilustrații: XI, 379 p. 94 illus., 40 illus. in color.
Dimensiuni: 178 x 254 x 22 mm
Greutate: 0.74 kg
Ediția:1st ed. 2021
Editura: Humana
Colecția Methods in Pharmacology and Toxicology
Seria Methods in Pharmacology and Toxicology

Locul publicării:New York, NY, United States

Cuprins

Cytochrome P450: In Vitro Methods and Protocols.- Characteristics of Major Drug Metabolizing Cytochrome P450 Enzymes.- Quantitative Determination of Cytochrome P450 Using LC/MS/MS.- Spectral Quantitation of Total Cytochrome P450 in Microsomes Using a Single-Beam Spectrophotometer.- Cytochrome P450 Enzyme Kinetics: Km and Vmax Determinations Using Metabolite Formation and Substrate Depletion Methods.- Cytochrome P450 Inhibition Assay Using Human Liver Microsomes.- Evaluation of Time-Dependent Cytochrome P450 Inhibition Using the Area Under the Curve Shift Method.- Assessing Cytochrome P450 Time Dependent Inhibition (IC50 Shift Assay) Using Both Diluted and Non-Diluted Methods.- Cytochrome P450 Gene Regulation: Reporter Assays to Assess Pregnane X Receptor (PXR, NR1I2) Activation.- Cytochrome P450 Gene Regulation: Reporter Assays to Assess Aryl Hydrocarbon Receptor (HLHE76, AhR) Activation and Antagonism.- Cytochrome P450 Gene Regulation: Reporter Assays to Assess Direct and Indirect Activation of Constitutive Androstane Receptor (CAR, NR1I3).- Determination of In Vitro Cytochrome P450 Induction Potential Using Cryopreserved Human Hepatocytes.- Assessing Cytochrome P450 3A (CYP3A) Induction and Suppression Using Primary Human Hepatocyte Spheroids.- Cytochrome P450-Mediated Metabolic Stability Assay in Liver Microsomes.- Assessment of Cytochrome P450 Metabolic Clearance Using Hepatocyte Suspension.- Cytochrome P450 Knock-Out Assay with Non-Selective Inhibitors.- Differentiation of Cytochrome P450-Mediated from Non-CYP-Mediated Metabolism: Aldehyde Oxidase and Xanthine Oxidoreductase.- In Vitro Reaction Phenotyping of Cytochrome P450 Enzymes.- Cytochrome P450-Mediated Drug Bioactivation Assay: An Untargeted High Resolution Accurate Mass LC/MS Assay.- Simultaneously Assessing Concentration Changes in 20 Biochemical Pathways as a Result of Drug Dosing and Cytochrome P450 and Non-Cytochrome P450-Mediated Metabolism: An Untargeted Metabolomics GC/MS Assay.-Simultaneously Assessing Concentration Changes in 17 Biochemical Pathways as a Result of Drug Dosing and Cytochrome P450 and Non-Cytochrome P450-Mediated Metabolism: A Quasi-Untargeted Metabolomics LC/MS Assay.- Assessing Amino Acid Concentration Changes as a Result of Drug Dosing and Cytochrome P450 and Non-Cytochrome P450-Mediated Metabolism.

Textul de pe ultima copertă

This collection explores detailed experimental protocols necessary for setting up a variety of in vitro cytochrome P450 (CYP) assays that are vital in selecting drug candidates in a drug discovery pipeline. Major factors affecting drug metabolism include CYP expression levels, kinetic parameters for individual CYP enzymes, CYP inhibition and induction, time-dependent inhibition (TDI), CYP stability, non-CYP stability, UDP-glucuronosyltransferases (UGT) stability, excretion mechanisms, and drug-drug interactions (DDI), all addressed in this volume. Written for the Methods in Pharmacology and Toxicology series, chapters include helpful background information on the in vitro assay, a list of all the materials, reagents, and equipment necessary to carry out the assay, a step-by-step protocol, notes containing common and unexpected experimental problems in the assay, as well as references containing important supplementary reading. 

Authoritativeand practical, Cytochrome P450: In Vitro Methods and Protocols serves as a key guide for researchers in the area of discovery and development of new medicines. 

Caracteristici

Includes cutting-edge techniques Provides the kind of detail necessary for reproducible results Contains key implementation advice from the experts