Crispr

Contributors Vii Preface xi S E C T I O N I Diversity of CRISPR-Cas Systems 1 1 CRISPR-Cas Systems: Core Features and Common Mechanisms 3 Rodolphe Barrangou, Luciano A. Marraffini, and Erik J. Sontheimer 2 Evolutionary Classification of CRISPR-Cas Systems 13 Kira S. Makarova, Yuri I. Wolf, and Eugene V. Koonin 3 Molecular Mechanisms of Type I CRISPR-Cas Systems 39 John van der Oost 4 Molecular Mechanisms of Type II CRISPR-Cas Systems 53 Tautvydas Karvelis and Virginijus Siksnys 5 Mechanism of Type III CRISPR-Cas Immunity 71 Luciano A. Marraffini 6 Type V CRISPR-Cas Systems 85 Morgan Quinn Beckett, Anita Ramachandran, and Scott Bailey 7 CRISPR-Cas13: Biology, Mechanism, and Applications of RNA-Guided, RNA-Targeting CRISPR Systems 99 Omar O. Abudayyeh and Jonathan S. Gootenberg S E C T I O N II CRISPR-Cas Biology 121 8 CRISPR-Cas, Horizontal Gene Transfer, and the Flexible (Pan) Genome 123 Uri Gophna 9 Evasion Tactics Manifested by Bacteriophages against Bacterial Immunity 131 Jenny Y. Zhang, Sutharsan Govindarajan, and Joseph Bondy-Denomy 10 Regulation of CRISPR-Cas Expression and Function 145 Leah M. Smith, Aroa Rey Campa, and Peter C. Fineran S E C T I O N III CRISPR-Based Technologies and Applications 163 11 Genome Editing with CRISPR-Cas Systems 165 Peter Lotfy and Patrick D. Hsu 12 Genetic and Epigenetic Modulation of Gene Expression by CRISPR-dCas Systems 195 Jasprina N. Noordermeer, Crystal Chen, and Lei S. Qi 13 CRISPR Screens 213 Jonathan D. D'Gama, Joseph S. Park, and Matthew K. Waldor 14 CRISPR-Based Antimicrobials 233 Justen Russell and David Bikard 15 Exploiting CRISPR-Cas Systems To Provide Phage Resistance in Industrial Bacteria 249 Rodolphe Barrangou and Avery Roberts 16 Recording Biological Information with CRISPR-Cas Systems 267 Mariia Y. Cherepkova, Tanmay Tanna, and Randall J. Platt Afterword 281 Index 283