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Chromaffin Cells de Ricardo Borges

Chromaffin Cells

Editat de Ricardo Borges
en Limba Engleză Paperback – 9 oct 2023
This volume covers the most up-to-date methods and techniques used to further the understanding of chromaffin cell biology and pharmacology. Chapters guide readers through the basic mechanisms that regulate the stimulus-secretion coupling, chromaffin, tumor-derived cell PC-12 , morphology, biochemistry, pharmacology, electrophysiology, and electrochemistry. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
  Authoritative and cutting-edge, Chromaffin Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. 
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Specificații

ISBN-13: 9781071626733
ISBN-10: 1071626736
Pagini: 388
Ilustrații: XIV, 373 p. 89 illus., 68 illus. in color.
Dimensiuni: 178 x 254 x 21 mm
Greutate: 0.73 kg
Ediția:1st ed. 2023
Editura: Humana
Locul publicării:New York, NY, United States

Cuprins

Rat pheochromocytoma PC12 cells in culture.- Adrenal grafts in the central nervous system: Chromaffin and chromaffin progenitor cell transplantation.- Immunocytochemistry of acutely isolated adrenal medullary chromaffin cells.- Transmission electron microscopy: a method for studying the adrenochromaffin cells.- Immunogold for protein location in chromaffin cells.- Visualization of exo- and endocytosis membrane dynamics with super-resolution STED microscopy.- Online detection of catecholamine release from the perfused rat adrenal gland.- On-line recording of perifused chromaffin cells.- Recording of chromaffin cell electrical activity in situ in acute adrenal slices.- Calcium imaging and amperometric recording in cultured chromaffin cells and adrenal slices from normotensive, Wistar Kyoto rats, and Spontaneously Hypertensive Rats.- Measurements of calcium in chromaffin cell organelles using targeted aequorins.- Quantification of secretory granule exocytosis by TIRF imaging and capacitance measurements.- Membrane capacitance measurements of stimulus-evoked exocytosis in adrenal chromaffin cell.- Quantal release analysis of electrochemically active molecules using single-cell amperometry.- Methodologies for detecting quantal exocytosis in adrenal chromaffin cells through diamond-based MEAs.- Vesicle collision protocols for the study of quantum size and exocytotic fraction released.- Patch Amperometry and Intracellular Patch Electrochemistry.- Artificial cells for dissecting exocytosis.- Isolation and purification of chromaffin granules from adrenal glands and cultured neuroendocrine cells.- Confocal microscopy studies of F-actin cytoskeleton distribution and dynamics using fluorescent LifeAct constructs in bovine adrenal chromaffin cells.- Unveiling the nanoscale dynamics of the exocytic machinery in chromaffin cells with single-molecule imaging.- Determinationof catecholamines in a small volume (25µl) of plasma from conscious mouse tail vein.- Quantification of chromogranin A and its fragments in biological fluids.- Cytotoxicity models in chromaffin cells to evaluate neuroprotective compounds.

Textul de pe ultima copertă

This volume covers the most up-to-date methods and techniques used to further the understanding of chromaffin cell biology and pharmacology. Chapters guide readers through the basic mechanisms that regulate the stimulus-secretion coupling, chromaffin, tumor-derived cell PC-12 , morphology, biochemistry, pharmacology, electrophysiology, and electrochemistry. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
  Authoritative and cutting-edge, Chromaffin Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. 

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts