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Chemosensitivity: Volume I: In Vitro Assays de Rosalyn D. Blumenthal

Chemosensitivity: Volume I: In Vitro Assays: Methods in Molecular Medicine, cartea 110

Editat de Rosalyn D. Blumenthal
en Limba Engleză Paperback – 10 noi 2010
A state-of-the art collection of readily reproducible laboratory methods for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors. Chemosensitivity, Volume 1: In Vitro Assays provides a panel of 16 in vitro measures of chemosensitivity in adherent and non-adherent cells for single agents and combinations of agents. In addition to immunohistochemical and imaging approaches, these assays include clonogenic, colorimetric, fluorometric, and physiological assays. Highlights include image analysis to assess drug sensitivity, high throughput approaches using green fluorescent protein, DIMSCAN (a microcomputer fluorescence-based assay), and the ChemoFx assay used in biotechnology. A companion volume, Volume 2: In Vivo Models, Imaging, and Molecular Regulators, provides protocols for classifying tumors into response categories and customizing chemotherapy regimens to individual patients.
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Specificații

ISBN-13: 9781617375088
ISBN-10: 161737508X
Pagini: 231
Ilustrații: XIV, 231 p.
Dimensiuni: 152 x 229 x 14 mm
Greutate: 0.37 kg
Ediția:Softcover reprint of hardcover 1st ed. 2005
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Medicine

Locul publicării:Totowa, NJ, United States

Public țintă

Professional/practitioner

Cuprins

An Overview of Chemosensitivity Testing.- Clonogenic Cell Survival Assay.- High-Sensitivity Cytotoxicity Assays for Nonadherent Cells.- Sulforhodamine B Assay and Chemosensitivity.- Use of the Differential Staining Cytotoxicity Assay to Predict Chemosensitivity.- Collagen Gel Droplet Culture Method to Examine In Vitro Chemosensitivity.- The MTT Assay to Evaluate Chemosensitivity.- Histoculture Drug Response Assay to Monitor Chemoresponse.- In Vitro Testing of Chemosensitivity in Physiological Hypoxia.- Chemosensitivity Testing Using Microplate Adenosine Triphosphate-Based Luminescence Measurements.- High-Throughput Technology.- Dimscan.- The ChemoFx® Assay.- Evaluating Response to Antineoplastic Drug Combinations in Tissue Culture Models.- Image Analysis Using the Fluorochromasia Assay to Quantify Tumor Drug Sensitivity.- Immunohistochemical Detection of Ornithine Decarboxylase as a Measure of Chemosensitivity Testing.- Immunohistochemistry of p53, Bcl-2, and Ki-67 as Predictors of Chemosensitivity.

Textul de pe ultima copertă

Chemosensitivity testing is an ex vivo means of determining or enhancing the cytotoxic and/or cytostatic, or apoptosis-inducing effects of anticancer drugs. In Chemosensitivity, leading researchers and physicians working in academia and biotech companies describe state-of-the-art laboratory methods for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors. Volume 1: In Vitro Assays provides a panel of 16 in vitro measures of chemosensitivity in adherent and nonadherent cells for single agents and combinations of agents. In addition to immunohistochemical and imaging approaches, these assays include clonogenic, colorimetric, fluorometric, and physiological assays. Highlights include image analysis to assess drug sensitivity, high throughput approaches using green fluorescent protein, DIMSCAN (a microcomputer fluorescence-based assay), and the ChemoFx assay used in biotechnology. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. The authors also provide guidance on how best to analyze the data derived from the protocols. A companion volume, Volume 2: In Vivo Models, Imaging, and Molecular Regulators, provides protocols for classifying tumors into response categories and customizing chemotherapy regimens to individual patients.
Cutting-edge and highly practical, the two volumes of Chemosensitivity provide a comprehensive collection of readily reproducible techniques for the in vitro and in vivo screening of new agents and a set of proven approaches to understand mechanistically why certain cancer cell lines (in vitro) of tumors (in vivo) are more, or less, sensitive to a particular agent.

Caracteristici

Includes supplementary material: sn.pub/extras