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Cereal Genomics

Cereal Genomics


en Limba Engleză Paperback – 23 aug 2016
​In Cereal Genomics: Methods and Protocols, expert researchers provides modern protocols for the analysis and manipulation of cereal genomes.  Techniques for isolation and analysis of DNA and RNA from both the vegetative tissues and from the more challenging seeds of cereals are described.  Tools for the isolation, characterization and functional analysis of cereal genes and their transcripts are detailed. Methods for molecular screening of cereals and for their genetic transformation are also covered. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cereal Genomics: Methods and Protocols provides a comprehensive resource for those studying cereal genomes.
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Specificații

ISBN-13: 9781493960392
ISBN-10: 1493960393
Pagini: 310
Ilustrații: X, 300 p. 39 illus., 29 illus. in color.
Dimensiuni: 178 x 254 x 17 mm
Greutate: 0.44 kg
Ediția:Softcover reprint of the original 1st ed. 2014
Editura: Humana Press Inc.
Locul publicării:Totowa, NJ, United States

Cuprins

DNA Extraction from Vegetative Tissue for Next Generation Sequencing  .- DNA Extraction from Rice Endosperm (Including a Protocol for Extraction of DNA from Ancient Seed Samples) .- RNA Extraction from Cereal Vegetative Tissue.- RNA Extraction from Developing or Mature Wheat Seeds.- cDNA Library Preparation.- Preparation of High Molecular Weight gDNA and Bacterial Artificial Chromosome (BAC) Libraries in Plants.- The Polymerase Chain Reaction (PCR) - General Methods.- Mutation and Mutation Screening.- The Quantitative Real-Time Polymerase Chain Reaction for the Analysis of Plant Gene Expression.- Cloning of DNA Fragments: Ligation Reactions in Agarose Gel.- Rapid Cloning of Genes and Promoters for Functional Analyses.- Genome Walking.- Functional Analysis by Protein Biochemistry.- Genomic Southern Blot analysis.- Northern Hybridization: A Proficient Method for Detection of Small RNAs and MicroRNAs.- Protein Blotting-Protocol for Beginners.- Genetic Transformation of Wheat via Particle Bombardment.- Sorghum Genetic Transformation by Particle Bombardment.- Genetic Transformation of Wheat via Agrobacterium-mediated DNA Delivery.- A Protocol for High-throughput Agrobacterium-mediated Barley Transformation.- Agrobacterium-mediated Transformation- Rice Transformation.- Agrobacterium-mediated Transformation of Maize (Zea mays) Immature Embryos.- A Technical Platform for PCR-based SNP Screening in Cereals and Other Crops.- A Method for Discovery of Genome-wide SNP Between Any Two Genotypes from Whole Genome Re-sequencing Data.

Textul de pe ultima copertă

In Cereal Genomics: Methods and Protocols,  expert researchers provides modern protocols for the analysis and manipulation of cereal genomes.  Techniques for isolation and analysis of DNA and RNA from both the vegetative tissues and from the more challenging seeds of cereals are described.  Tools for the isolation, characterization and functional analysis of cereal genes and their transcripts are detailed. Methods for molecular screening of cereals and for their genetic transformation are also covered.  Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and practical, Cereal Genomics: Methods and Protocols provides a comprehensive resource for those studying cereal genomes.

Caracteristici

Is a comprehensive resource for studying cereal genomes Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Notă biografică

This book is the first of its kind, where twenty chapters written by experts of international repute, cover all aspects of cereal genomics research. In recent years genomics has become a thrust area of research in life sciences, and cereals, being the most important group of crops, their genomes have been subjected to intensive/extensive analyses. The knowledge and the products generated through cereal genomics research are already being used for plant breeding through both, the marker assisted selection (MAS) and transgenic cereals. The areas covered in this book include, development and use of all kinds of molecular markers (including SNPs), construction and use of molecular maps, study of population genetics and domestication of cereals using tools of genomics research, structural and functional genomics (including whole genome sequencing in Arabidopsis and rice), comparative genomics, QTLs (including epistatic and e-QTLs) and genes for resistance against biotic and abiotic stresses, marker assisted selection (MAS), map-based cloning of genes/QTLs and the use of genomics research for crop improvement. The book, therefore, should prove useful not only for students and teachers, but also for the young research workers, who are starting their research career in the field of cereal genomics.