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Carbohydrate-Based Vaccines: Methods and Protocols de Bernd Lepenies

Carbohydrate-Based Vaccines: Methods and Protocols: Methods in Molecular Biology, cartea 1331

Editat de Bernd Lepenies
en Limba Engleză Paperback – 15 oct 2016
This volume summarizes current cutting-edge methods related to carbohydrate-based vaccines, from the identification of a suitable carbohydrate antigen via the preparation of glycoconjugate vaccines to the characterization of vaccine candidates for their use in pre-clinical and clinical studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Carbohydrate-Based Vaccines: Methods and Protocols aims to be a valuable manual that will facilitate research in the field of carbohydrate-based vaccines.
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Specificații

ISBN-13: 9781493949199
ISBN-10: 1493949195
Pagini: 255
Ilustrații: XI, 255 p. 73 illus., 21 illus. in color.
Dimensiuni: 178 x 254 x 19 mm
Ediția:Softcover reprint of the original 1st ed. 2015
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Carbohydrate-based Vaccines: An Overview.- Glycans as Vaccine Antigens and Adjuvants: Immunological Considerations.- The Glycan Array Platform as a Tool to Identify Carbohydrate Antigens.- Antibody-carbohydrate Recognition from Docked Ensembles using the AutoMap Procedure.- Generation of Monoclonal Antibodies against Defined Oligosaccharide Antigens.- Murine Whole-Blood Opsonophagocytosis Assay to Evaluate Protection by Antibodies Raised Against Encapsulated Extracellular Bacteria.- Determination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility.- N-Glycosylation Fingerprinting of Viral Glycoproteins by xCGE-LIF.- Temporary Conversion of Protein Amino Groups To Azides: A Synthetic Strategy For Glycoconjugate Vaccines.- Gold Nanoparticles as Carriers For Synthetic Glycoconjugate Vaccines.- Identification and Characterization of Carbohydrate-Based Adjuvants.- Characterization of Carbohydrate Vaccines by NMR Spectroscopy Francesco Berti and Neil Ravenscroft.- Characterisation of Capsular Polysaccharides and Their Glycoconjugates by Hydrodynamic Methods.- Glycoconjugate Vaccines: The Regulatory Framework.

Textul de pe ultima copertă

 This volume summarizes current cutting-edge methods related to carbohydrate-based vaccines, from the identification of a suitable carbohydrate antigen via the preparation of glycoconjugate vaccines to the characterization of vaccine candidates for their use in pre-clinical and clinical studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Carbohydrate-Based Vaccines: Methods and Protocols aims to be a valuable manual that will facilitate research in the field of carbohydrate-based vaccines.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts