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Blood-Brain Barrier de Tatiana Barichello

Blood-Brain Barrier: Neuromethods

Editat de Tatiana Barichello
en Limba Engleză Hardback – 23 mai 2025
Chapters guide readers through the physiology of BBB, in vitro cell models to study the BBB, in vivo and ex vivo models to evaluate BBB, permeability, influx, efflux transportation, drug delivery through the BBB, invasive and non-invasive imaging techniques to study BBB;
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Specificații

ISBN-13: 9781071644737
ISBN-10: 1071644734
Pagini: 408
Dimensiuni: 183 x 260 x 28 mm
Greutate: 0.92 kg
Ediția:Second Edition 2025
Editura: Springer Us
Seria Neuromethods

Cuprins

An Overview of the Blood-Brain Barrier.- Methods of Delivering Molecules Through the Blood-Brain Barrier for Brain Diagnostics and Therapeutics.- Culturing of Rodent Brain Microvascular Endothelial Cells for In Vitro Modelling of the Blood-Brain Barrier.- In Vitro BBB Models: Working with Static Platforms and Microfluidic Systems.- In Vitro Cell Models of the Human Blood-Brain Barrier: Demonstrating the Beneficial Influence of Shear Stress in Brain Microvascular Endothelial Cell Phenotype.- Transepithelial/Transendothelial Electrical Resistance (TEER) to Measure the Integrity of Blood-Brain Barrier.- Cell Penetrating Peptides as Theranostics against Impaired Blood-Brain barrier Permeability: Implications for Pathogenesis and Therapeutics Treatment of Neurodegenerative Disease.- Microbial Translocation of the Blood-Brain Barrier.- Transport across the Choroid Plexus: How to Culture Choroid Plexus Cells and Establish a Functional Assay System.- Drosophila as a Model to Study the Blood-Brain Barrier.- Zebrafish (Danio rerio) as a Viable Model to Study the Blood-Brain Barrier.- Evans Blue-Albumin as a Marker to Evaluate Blood-Brain Barrier Integrity in Neonatal and Adult Rodents.- Experimental Tools to Study the Regulation and Function of the Choroid Plexus.- Techniques for Evaluating Efflux Transport of Radiolabeled Drugs and Compounds from the Cerebrospinal Fluid acorss the Blood-Cerebrospinal Fluid Barrier.- In Vivo Analysis to Study Transport across the Blood-Retinal Barrier.- Increasing BBB Permeability via Focused Ultrasound: Current Methods in Pre-Clinical Research.- Evaluation of Blood-Brain Barrier Permeability and Integrity in Juvenile Rodents: Dynamic Contrast-Enhanced (DCE), Magnetic Resonance Imaging (MRI), and Evan’s Blue Extravasation.- Recording Leukocyte Rolling and Adhesion on Meningeal Vessels by Intravital Microscopy.- Molecular Imaging of Blood-Brain Barrier Permeability in Preclinical Models Using PET and SPECT.- Biomarkers for Microvascular Proteins Detection: Blood-Brain Barrier Injury and Damage Measurement.

Textul de pe ultima copertă

This volume focuses on experimental research with applicable models to study physiology, biochemistry, and molecular biology of the blood-brain barrier (BBB). This book is organized into six parts: Part One is an overview of the physiology of BBB; Part Two explores in vitro cell models to study the BBB; Part Three discusses techniques in vivo and ex vivo models to evaluate BBB in Drosophila melanogaster, Zebrafish, and rodents; Part Four looks at permeability, influx, efflux transportation, and drug delivery through the BBB; Part Five talks about various invasive and non-invasive imaging techniques to study BBB; and Part Six describes how molecular biomarkers are used to look at the integrity or dysfunction of the BBB. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. 

Cutting-edge and thorough, Blood-Brain Barrier is avaluable resource to aid both novice and experienced investigators with performing experiments using new and classic translational approaches.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts