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Adhesion Protein Protocols: Methods in Molecular Biology, cartea 370

Editat de Amanda S. Coutts
en Limba Engleză Hardback – 26 feb 2007
Cellular adhesion is a fundamental process that influences numerous biological activities such as morphogenesis, cell motility and division, as well as signalling. In addition, adhesion is a process important not only in normal physiology and development, but also in disease states such as tumourigenesis, cardiovascular disease, inflammation and infection. There are a plethora of proteins involved in adhesion-related events with a huge diversity in function. As a result, a wide variety of techniques exist to study adhesion related proteins and processes. In Adhesion Protein Protocols, Third Edition, chapters cover techniques to gain insight into the complex and incompletely understood processes that are involved in cellular adhesion. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
 
Authoritative and easily accessible, Adhesion Protein Protocols, Third Edition will be useful for both those new to the field of adhesion protein research as well as the more experienced scientist.
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Specificații

ISBN-13: 9781588295330
ISBN-10: 1588295338
Pagini: 248
Ilustrații: X, 234 p. 54 illus., 2 illus. in color.
Dimensiuni: 155 x 235 x 21 mm
Greutate: 0.59 kg
Ediția:2nd ed. 2007
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Research

Cuprins

Manipulation of Cell-Cell Adhesion Using Bowtie-Shaped Microwells.- Separation of Cell-Cell Adhesion Complexes by Differential Centrifugation.- Analysis of Neutrophil Chemotaxis.- Analysis of Leukocyte Migration Through Monolayers of Cultured Endothelial Cells.- Biochemical Purification of Pseudopodia from Migratory Cells.- Dynamic Assessment of Cell-Matrix Mechanical Interactions in Three-Dimensional Culture.- Quantitative Analyses of Cell Adhesion Strength.- Using RNA Interference to Knock Down the Adhesion Protein TES.- Assaying Calpain Activity.- Analysis of Focal Adhesions and Cytoskeleton by Custom Microarray.- Proteomic Analysis of Cell Surface Membrane Proteins in Leukemic Cells.- Bioinformatic Analysis of Adhesion Proteins.- Analysis of Integrin Dynamics by Fluorescence Recovery After Photobleaching.- Double-Hydrogel Substrate as a Model System for Three-Dimensional Cell Culture.- In Vitro Actin Assembly Assays and Purification From Acanthamoeba.

Caracteristici

Novel techniques for studying cell-cell adhesion The latest methods for laboratory use of microarray techniques Adhesion protein proteomics Includes supplementary material: sn.pub/extras

Textul de pe ultima copertă

Cellular adhesion is a fundamental process that influences numerous biological activities such as morphogenesis, cell motility and division, as well as signalling. In addition, adhesion is a process important not only in normal physiology and development, but also in disease states such as tumourigenesis, cardiovascular disease, inflammation and infection. There are a plethora of proteins involved in adhesion-related events with a huge diversity in function. As a result, a wide variety of techniques exist to study adhesion related proteins and processes. In Adhesion Protein Protocols, Third Edition, chapters cover techniques to gain insight into the complex and incompletely understood processes that are involved in cellular adhesion. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
 
Authoritative and easily accessible, Adhesion Protein Protocols, Third Edition will be useful for both those new to the field of adhesion protein research as well as the more experienced scientist.