Activity-Based Proteomics
Editat de Herman S. Overkleeft, Bogdan I. Floreaen Limba Engleză Paperback – 10 iul 2018
Authoritative and cutting-edge, Activity-Based Proteomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.
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Specificații
ISBN-13: 9781493982004
ISBN-10: 1493982001
Pagini: 236
Ilustrații: XI, 222 p. 47 illus., 18 illus. in color.
Dimensiuni: 178 x 254 x 13 mm
Greutate: 0.51 kg
Ediția:Softcover reprint of the original 1st edition 2017
Editura: Humana
Locul publicării:New York, NY, United States
ISBN-10: 1493982001
Pagini: 236
Ilustrații: XI, 222 p. 47 illus., 18 illus. in color.
Dimensiuni: 178 x 254 x 13 mm
Greutate: 0.51 kg
Ediția:Softcover reprint of the original 1st edition 2017
Editura: Humana
Locul publicării:New York, NY, United States
Cuprins
Activity-Based Protein Profiling – From Chemical Novelty to Biomedical Stalwart.- A Quantitative Mass-Spectrometry Platform to Monitor Changes in Cysteine Reactivity.- Activity-Based Protein Profiling with Natural Product-Derived Chemical Probes in Human Cell Lysates.- Inhibitor Discovery by Convolution Activity-Based Protein Profiling.- Activity-Based Protein Profiling in Bacteria.- Activity-Based Lipid Esterase Profiling of M. Bovis BCG at Different Metabolic States Using Tetrahydrolipstatin (THL) as Bait.- Comparative Activity-Based Flavin-Dependent Oxidase Profiling.- Activity-Dependent Photoaffinity Labelling of Metalloproteases.- Profiling the Activity of Deubiquitinating Enzymes using Chemically Synthesized Ubiquitin-Based Probes.- Profiling Protein Sumoylation using Biochemical and Mass Spectrometry-Based Research Tools.- Live Cell Imaging and Profiling of Cysteine Cathepsin Activity using a Quenched Activity-Based Probe.- Competitive Activity-Based Protein Profiling Of Serine Hydrolases: A Case Study on DAGL-Alpha.- A Protocol for Protein Profiling using Chemoselective Cleavable Linker Probes in Semi-Permeabilized Cells.- Cleavable Linkers in Chemical Proteomics Applications.- Two-Step Activity-Based Protein Profiling With the Proteasome System as Model of Study.
Caracteristici
Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras