Weary of her life in English society, during 1878-1879 Dixie travelled with her husband, two of her brothers and Julius Beerbohm in Patagonia in South America. There, she hunted big game and ate it with gusto. On one occasion, while riding on the prairie, her party was overtaken by a huge prairie fire, and her horse bolted with her. On her return to England, Dixie wrote her book Across Patagonia, which discussed Dixie's observations of the country and its inhabitants. Lady Dixie also shared her observations of Patagonia with Charles Darwin. Lady Dixie sent Darwin a copy of Across Patagonia; Darwin's copy of this book is part of the Library of Charles Darwin located in the Rare Books Room of Cambridge University Library. A hotel at Puerto Natales in the Chilean part of Patagonia is named the Hotel Lady Florence Dixie in her honour.