Cantitate/Preț
Produs
Total produse
Vezi coșul
A Laboratory Guide to Glycoconjugate Analysis de P. Jackson

A Laboratory Guide to Glycoconjugate Analysis: Biomethods

Editat de P. Jackson, J.T. Gallagher
en Limba Engleză Paperback – 12 iun 2012
18. 2 Principle of FACE/Gel Retardation Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 349 18. 3 Labelling of Oligosaccharides with ANTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 350 18. 4 Screening of Carbohydrate Ligands for Proteins . . . . . . . . . . . . . . . . . . . . . . . . . . . 352 18. 5 Measurement of Binding Constant for the Interaction Between Protein and ANTS-Labelled Carbohydrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 355 18. 6 Measurement of Binding Constant for the Interaction Between Protein and Native Carbohydrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 357 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 360 ~ The Application of Capillary Affinity Electrophoresis to the Analysis _ of Carbohydrate-Protein Interactions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 361 19. 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 361 19. 2 Principle of CAE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 363 19. 3 Determination of Association Constants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 364 19. 4 Technical Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 366 General considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 366 19. 5 Limitations of the Technique . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . 370 19. 6 Application of CAE to the Analysis of Carbohydrate-Protein Interactions . . . . . . 371 19. 7 Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 375 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 377 20. 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 379 Definitions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 380 20. 2 Technical Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 381 20. 3 Sample Detection and Sample Recovery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 Autoradiography and staining . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 Sample detection by blotting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 Semipreparative ACE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 390 20. 4 Analysis of Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 391 Measuring sample mobilities - calculating a retardation coefficient . . . . . . . . . . . . 391 Graphical analysis of data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 392 Interpreting ACE patterns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 393 Reverse ACE . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 395 20. 5 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 397 Acknowledgements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 398 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 398 Subject Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 399 XII List of Contributors Nebojsa Avdalovic John T. Gallagher Dionex Corporation Cancer Research Campaign Department of Medical Oncology 445 Lakeside Drive University of Manchester Sunnyvale, CA 94086 Christie CRC Research Centre Klaus Biemann Wilmslow Road Department of Chemistry Manchester M20 4BX Massachusetts Institute of Technology UK Cambridge, MA 02139-4307 USA Geoffrey R.
Citește tot Restrânge

Din seria Biomethods

  • 15% Microsystem Technology: A Powerful Tool for Biomolecular Studies
    Preț: 63438 lei
  • Analytical Graphite Furnace Atomic Absorption Spectrometry
    Preț: 37914 lei
  • 15% Methods in Comparative Psychoacoustics
    Preț: 62134 lei
  • 15% The Resonant Recognition Model of Macromolecular Bioactivity
    Preț: 63775 lei
  • A Laboratory Guide for Cellular and Molecular Plant Biology
    Preț: 38172 lei
  • A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
    Preț: 37639 lei
  • A laboratory guide for in vivo studies of DNA methylation and protein/DNA interactions
    Preț: 37215 lei
  • A Laboratory Guide to Genomic Sequencing
    Preț: 37123 lei
  • A Laboratory Guide to In Vitro Transcription
    Preț: 36908 lei
  • A Laboratory Guide to In Vitro Studies of Protein-DNA Interactions
    Preț: 37805 lei

Preț: 62526 lei

Preț vechi: 73560 lei
-15%

Puncte Express: 938

Carte tipărită la comandă

Livrare economică 08-22 septembrie

Livrare prin curier în România Termenul estimat este afișat lângă disponibilitate.
Transport gratuit pentru acest produs Plată online sau ramburs, în funcție de opțiunile comenzii.
Retur gratuit în 14 zile Comandă securizată și suport în română.
 Adaugă în coș

Wish list
Gift list
Am citit!
Adaugă în listă

Specificații

ISBN-13: 9783034873901
ISBN-10: 3034873905
Pagini: 436
Ilustrații: XXII, 410 p. 724 illus.
Dimensiuni: 170 x 244 x 23 mm
Greutate: 0.69 kg
Ediția:1997
Editura: Birkhäuser Basel
Colecția Birkhäuser
Seria Biomethods

Locul publicării:Basel, Switzerland

Public țintă

Research

Cuprins

1 High-Performance Liquid Chromatography of Derivatized and Non-Derivatized Oligosaccharides: A Review.- 2 Detection of the Presence of Carbohydrates in Glycoproteins.- 3 HPAE-PAD Monosaccharide and Oligosaccharide Analysis of Glycoproteins Electrotransferred onto Polyvinylidene Fluoride Membranes.- 4 Determination of the Disaccharide Composition of Glycosaminoglycans: Comparison of Chemical and Enzymatic Scission.- 5 Mass Spectrometric Analysis of Highly Acidic Polysaccharides.- 6 Analysis of N-Glycans by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry.- 7 Polyacrylamide Gel Electrophoresis of Fluorophore-Labelled Reducing Saccharides: A Review.- 8 Carbohydrate Analysis with Capillary Electrophoresis.- 9 Oligosaccharide Profiling of Keratan Sulphate.- 10 Analysis of the Structure of Heparin and Heparan Sulfate by High-Resolution Separation of Oligosaccharides.- 11 HPLC Strategies for Profiling and Sequencing Oligosaccharides.- 12 Enzymatic Sequence Analysis of Glycoprotein Glycans.- 13 Exosequencing of Heparan Sulphate/Heparin Saccharides Using Lysosomal Enzymes.- 14 The Application of Two-Dimensional Capillary Electrophoresis to the Identification of Glycan Structures.- 15 The Application of Three-Dimensional HPLC to the Identification of N-linked Oligosaccharide Structures.- 16 Simultaneous Fluorescent Labelling and Biotinylation of Oligosaccharides: A Versatile Approach to the Analysis of Oligosaccharide Structure and Function.- 17 Preparation of Neoglycolipids for Structure and Function Assignments of Oligosaccharides.- 18 Analysis of Protein — Carbohydrate Interactions by FACE/Gel Retardation Assay.- 19 The Application of Capillary Affinity Electrophoresis to the Analysis of Carbohydrate-Protein Interactions.- 20 Analysis ofProtein-Glycosaminoglycan Interactions by Affinity Co-Electrophoresis.