This report, A Field Manual for the Use of Antimycin A for Restoration of Native Fish Populations, provides protocols for the use of antimycin A which have been successfully used in restoration efforts within units of the national park system and in national forest and state projects. This document is based upon experiences of biologists from the National Park Service (NPS), the U.S. Fish and Wildlife Service (USFWS) and New Mexico Department of Game and Fish. The authors' hope that the information included in the manual will be applicable to any application of antimycin for the restoration of native fish populations and that it provides fisheries managers with established protocols for safe, effective, and lawful restoration projects utilizing antimycin A.