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2D and 3D Cellular Screening Models and AI Guided Analysis de Lorenzo Galluzzi

2D and 3D Cellular Screening Models and AI Guided Analysis: Methods in Cell Biology, cartea 204

Lorenzo Galluzzi Oliver Kepp, Guido Kroemer
en Limba Engleză Hardback – mai 2026
2D and 3D Cellular Screening Models and AI Guided Analysis provides a comprehensive overview of the latest advancements in cellular screening models, with a particular focus on both 2D and 3D systems. It explores the development, application, and comparative benefits of 2D monolayer cultures and 3D organoids, spheroids, and tissue-engineered constructs for biomedical research and drug discovery.

  • Detailed methodologies and protocols for developing both 2D monolayer and 3D spheroid/organoid cellular screening models
  • In-depth comparison of advantages, limitations, and applications of 2D vs. 3D cellular models in research and drug discovery
  • Description of high-throughput and high-content screening methods for assessing cellular responses
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Specificații

ISBN-13: 9780443428517
ISBN-10: 0443428514
Pagini: 318
Dimensiuni: 152 x 229 mm
Editura: ELSEVIER SCIENCE
Seria Methods in Cell Biology

Cuprins

1. Preface
Guido Kroemer and Oliver Kepp
2. High throughput CellTiter-glo viability assessment
Dana Alawieh and Aline Renneville
3. Assessing viability of cancer cell spheroids in 3D drug screening
Oliver Kepp and Marion Leduc
4. 3D killing assay of cancer spheroids by cytotoxic T lymphocytes in anchored microfluidic droplets
Charles Baroud
5. Use of micropatterning for 3D cell culture
Emna Ouni and Kristine Schauer
6. Use of microfluidic chip devices for assessing the effect of radiation on 3D cell culture models
Philip Wong, Rodin Chermat, Elena Refet-Mollof, Maryam Ziaee, Julie Lafontaine and Thomas Gervais
7. A 3D model for assessing regulated cell death modalities
Dmitri Krysko and Robin Demuynck
8. YOLO detection model for Cell Viability assessment
Felix Peyre
9. Bioinformatics and AI/ML approaches using multi-omics data to accelerate diagnosis and delivery of precision care
Zeeshan Ahmed, Kritika Singh, Sarah Usman, Saman Zeeshan, Naveena Yanamala, Vikram Bhise, Mark Nichols, Sabahat Bokhari and Partho Sengupta
10. Automated image analysis and machine learning for the assessment of mitochondrial membrane potential
Marta Giacomello, Camilla Bean, Federica Dal bello, Emad Norouzi Esfahani, Alessio Gianelle, Saradha Ramesh, Luca Persano, Elena Rampazzo and Caterina Vianello
11. Assessing cancer cell heterogeneity in patient-derived tumoroids
Flora Doffe
12. Assessment of lysosomal drug sequestration and release using fluorescence microscopy
Karla Alvarez, Mojgan Djavaheri-Mergny, Guido Kroemer, Lucille Ferret, Samy Dehissi and Jean Guillon
13. Assessment of T cell toxicity in ex-vivo models of cancer
Emmanuel intégrative des tumeurs et immunothérapie Donnadieu and Alice Machado
14. Three-dimensional Organoid Screening using Leica Thunder Microscope
Manoliu Tudor
15. Using vascularized organoids-on-chip for assessing systemic drug delivery
Xavier Gidrol, Emily Tubbs, Fabrice P Navarro and Yves Fouillet
16. Quantitative assessment of nanoparticle uptake and trafficking in 3D cell culture models
Meritxell B. Cutrona
17. Fluorescence microscopic quantitation of lysosomal cholesterol accumulation induced by cationic amphiphilic drugs
Mojgan Djavaheri-Mergny, Karla Alvarez, Samy Dehissi, Lucille Ferret and Guido Kroemer