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Lipids de Gilbert Di Paolo

Lipids: Methods in Cell Biology

Gilbert Di Paolo, Markus R. Wenk
en Limba Engleză Hardback – 28 mar 2012
Lipids are a broad group of naturally occurring molecules which includes fats, waxes, sterols, fat-soluble vitamins (such as vitamins A, D, E and K), monoglycerides, diglycerides, phospholipids, and others. The main biological functions of lipids include energy storage, as structural components of cell membranes, and as important signaling molecules.This volume of Methods in Cell Biology covers such areas as Membrane structure and dynamics, Imaging, and Lipid Protein Interactions. It will be an essential tool for researchers and students alike.


  • Covers such areas as membrane structure and dynamics, imaging, and lipid protein interactions
  • An essential tool for researchers and students alike
  • International authors
  • Renowned editors
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Specificații

ISBN-13: 9780123864871
ISBN-10: 0123864879
Pagini: 528
Dimensiuni: 191 x 235 x 38 mm
Editura: ELSEVIER SCIENCE
Seria Methods in Cell Biology

Public țintă

Researchers and students in cell, molecular and developmental biology

Cuprins

Membrane Dynamics and Reconstitution Assays:
  1. Supported native plasma membranes as platforms for the reconstitution and visualization of endocytic membrane budding
  2. Studying lipids involved in the endosomal pathway
  3. Studying in vitro membrane curvature recognition by proteins and its role in vesicular trafficking
  4. Reconstituting Multivesicular Body Biogenesis with purified components
  5. Approaches to the study of Atg8-mediated membrane dynamics in vitro
  6. Reconstitution assay system for ceramide transport with semi-intact cells
  7. Visualizing mitochondrial lipids and fusion events in mammalian cellsLipid Metabolism and Signaling:
  8. Targeted and non-targeted analysis of membrane lipids using mass spectrometry
  9. Modulation of host phosphoinositide metabolism during Salmonella invasion by the type III secreted effector SopB
  10. Acute Manipulation of Phosphoinositide Levels in Cells
  11. Regulation of phosphoinositide-metabolizing enzymes by clathrin coat proteins
  12. Phosphoinositides at the neuromuscular junction of Drosophila melanogaster: a genetic approach.
  13. Devising powerful Genetics, Biochemical and Structural tools in the Functional Analysis of Phosphatidylinositol Transfer Proteins (PITPs) across diverse species
  14. Genome-wide screens for gene products regulating lipid droplet dynamics.Imaging:
  15. The Three Dimensionality of Cell Membranes: Lamellar to Cubic Membrane Transition as Investigated by Electron Microscopy
  16. Quantitative imaging of lipid metabolism in yeast: from 4D analysis to high content screens of mutant libraries
  17. Analysis of cholesterol trafficking with fluorescent probes
  18. Fluorescence Correlation Methods for Imaging Cellular Behavior of Sphingolipid-Interacting Probes
  19. Monitoring Phospholipid Dynamics during Phagocytosis: Application of Genetically-Encoded Fluorescent Probes
  20. Genetically encoded probes for phosphatidic acid