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Understanding PCR: A Practical Bench-Top Guide de Rowena Jenkins

Understanding PCR: A Practical Bench-Top Guide

Autor Rowena Jenkins, Josie Parker
en Limba Engleză Paperback – mai 2027
Understanding PCR: A Practical Bench-Top Guide delivers a comprehensive resource offering researchers the information they need to plan their polymerase chain reactions, from reagents and conditions to analysis and beyond. This updated edition covers a broad spectrum of topics including laboratory preparation, primer design, DNA analysis, quantitative PCR, cloning, protein expression, and the latest applications. The book equips researchers with the knowledge to confidently design and execute PCR experiments, enhancing reproducibility and efficiency. By integrating updated techniques and practical insights, this revised reference supports researchers, laboratory professionals, and students working across molecular biology and related fields.

  • Includes step-by-step guidance for preparing and conducting PCR experiments
  • Features a new chapter on the use of PCR for CRISPR-Cas9 genome editing
  • Delivers updated resources for reagents and supplier selection
  • Includes troubleshooting tips, complemented by blank pages for note-taking to facilitate practical use
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Specificații

ISBN-13: 9780443525261
ISBN-10: 0443525269
Pagini: 150
Dimensiuni: 152 x 229 mm
Ediția:2
Editura: ELSEVIER SCIENCE

Cuprins

1. Things to consider before preparing to undertake your first polymerase chain reaction
2. Designing and ordering your polymerase chain reaction primers
3. Analyzing your template DNA and/or PCR product
4. Quantitative PCR: things to consider
5. Carrying Out Q-PCR
6. Using PCR for cloning and protein expression
7. Using PCR for CRISPR-Cas9 genome editing

Notă biografică

Rowena Jenkins, PhD, is currently a medical writer at Health Economics and Outcomes Research Ltd, UK. Prior to this she served as lecturer in Microbiology and Infectious Disease in the College of Medicine at Swansea University, UK. Her research team are focused on the effect of novel antimicrobial agents on pathogenic bacteria found in clinical infections, particularly chronic infections such as those seen in diabetic foot ulcers and cystic fibrosis lungs infection, and the impact on the microbiome within those conditions. Dr. Jenkins also has an interest in the potential for natural antimicrobial agents to improve the efficacy of conventional antibiotics against antibiotic resistant bacteria and biofilms. Dr. Jenkins serves as a section editor for the Journal of Medical Microbiology, and has co-authored two books with Elsevier, including the first edition of "Understanding PCR".