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Two-Component Signaling Systems, Part B de Melvin I. Simon

Two-Component Signaling Systems, Part B: Methods in Enzymology, cartea 423

Melvin I. Simon, Brian Crane, Alexandrine Crane
en Limba Engleză Hardback – 26 iul 2007
Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system."
Sections in Two-Component Signaling Systems, Part B include:
  • Structural Approaches
  • Reconstitution of Heterogeneous Systems
  • Intracellular Methods and Assays
  • Genome-Wide Analyses of Two-Component Systems


  • Presents detailed protocols
  • Includes troubleshooting tips
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Specificații

ISBN-13: 9780123738523
ISBN-10: 0123738520
Pagini: 648
Ilustrații: Illustrated
Dimensiuni: 152 x 229 x 43 mm
Greutate: 1.67 kg
Editura: ELSEVIER SCIENCE
Seria Methods in Enzymology

Public țintă

Biochemists, geneticists, and molecular biologists.

Cuprins

Section I: Structural Approaches
[1]: The PICM Chemical Scanning Method for Identifying Domain–Domain and Protein–Protein Interfaces: Applications to the Core Signaling Complex of E. coli Chemotaxis
[2]: Use of Site-Directed Cysteine and Disulfide Chemistry to Probe Protein Structure and Dynamics: Applications to Soluble and Transmembrane Receptors of Bacterial Chemotaxis
[3]: Measuring Distances by Pulsed Dipolar ESR Spectroscopy: Spin-Labeled Histidine Kinases
[4]: Rigid Body Refinement of Protein Complexes with Long-Range Distance Restraints from Pulsed Dipolar ESR
[5]: TonB/TolA Amino-Terminal Domain Modeling
[6]: Functional Dynamics of Response Regulators Using NMR Relaxation Techniques
[7]: The Design and Development of Tar-EnvZ Chimeric Receptors
[8]: Functional and Structural Characterization of EnvZ, an Osmosensing Histidine Kinase of E. coli
[9]: Light Modulation of Histidine-Kinase Activity in Bacterial Phytochromes Monitored by Size Exclusion Chromatography, Crosslinking, and Limited Proteolysis
[10]: A Temperature-Sensing Histidine Kinase—Function, Genetics, and Membrane Topology
[11]: The Regulation of Histidine Sensor Kinase Complexes by Quorum Sensing Signal Molecules
Section II: Reconstitution of Heterogeneous Systems
[12]: Liposome-Mediated Assembly of Receptor Signaling Complexes
[13]: Analyzing Transmembrane Chemoreceptors Using In Vivo Disulfide Formation Between Introduced Cysteines
[14]: Using Nanodiscs to Create Water-Soluble Transmembrane Chemoreceptors Inserted in Lipid Bilayers
[15]: Assays for CheC, FliY, and CheX as Representatives of Response Regulator Phosphatases
[16]: Genetic Dissection of Signaling Through the Rcs Phosphorelay
Section III: Intracellular Methods and Assays
[17]: In Vivo Measurement by FRET of Pathway Activity in Bacterial Chemotaxis
[18]: In Vivo and In Vitro Analysis of the Rhodobacter sphaeroides Chemotaxis Signaling Complexes
[19]: In Vivo Crosslinking Methods for Analyzing the Assembly and Architecture of Chemoreceptor Arrays
[20]: A “Bucket of Light” for Viewing Bacterial Colonies in Soft Agar
[21]: Phenotypic Suppression Methods for Analyzing Intra- and Inter-Molecular Signaling Interactions of Chemoreceptors
[22]: Single-Cell Analysis of Gene Expression by Fluorescence Microscopy
Section IV: Genome-Wide Analyses of Two-Component Systems
[23]: Two-Component Systems of Mycobacterium tuberculosis—Structure-Based Approaches
[24]: Transcriptomic Analysis of ArlRS Two-Component Signaling Regulon, a Global Regulator, in Staphylococcus aureus
[25]: Global Analysis of Two-Component Gene Regulation in H. pylori by Mutation Analysis and Transcriptional Profiling
[26]: Phosphotransfer Profiling: Systematic Mapping of Two-Component Signal Transduction Pathways and Phosphorelays
[27]: Identification of Histidine Phosphorylations in Proteins Using Mass Spectrometry and Affinity-Based Techniques
Subject Index
Author Index