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The Polymerase Chain Reaction de Kary B. Mullis

The Polymerase Chain Reaction

Editat de Kary B. Mullis, Richard Gibbs, F. Ferre
en Limba Engleză Hardback – 31 mar 1994
This text on the polymerase chain reaction (PCR) examines the most up-to-date methodological protocols. Included are new techniques and enhanced methods that show the novice and experienced PCR user how to optimize results. Future enhancements and new potential uses of PCR are discussed.
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Specificații

ISBN-13: 9780817636074
ISBN-10: 0817636072
Pagini: 550
Dimensiuni: 190 x 265 x 30 mm
Greutate: 1.05 kg
Editura: Birkhauser
Locul publicării:Boston, MA, United States

Cuprins

Methodology; manipulation of DNA, K. Hayashi; cloning PCR products, M. Frohman; multiplex PCRs, J.S. Chamberlain & J.R. Chamberlain; preparation of nucleic acids, D. Shibata; amplification of viral DNA and viral host cell mRNAs in situ, J. Embretson et al; quantitation - an overview, F. Ferre et al; quantification of DNAs by the polymerase chain using an internal control, D.M. Coen; RT-PCR and mRNA quantitation, J. Chelly and A. Kahn; analysis of T-cell repertoires by PCR, D. Spinella and J.M. Robertson; ultra-sensitive non-radioactive detection of PCR products - an overview, R.H. Tullis; fluorescent detection methods for PCR analysis, M.J. Heller; enzyme-labeled oligonucleotides, E. Tu & E. Jablonski; application of the hybridization protection assay to PCR, N.C. Nelson & S.H. McDonough; instrumentation - an overview, C. Oste; rapid cycle DNA amplification, C.T. Wittwer et al; automating PCR, H.R. Gardner; sequencing - an overview, R.A. Gibbs; and applications chapters on PCR and cancer, diagnostics, gene therapy, plant biology, forensics, and much more.