This book contains 10 Chapters divided into three Sections. Section A covers synthesis of biopolymers. Lignocellulosic feedstock contains cellulose, hemicellulose, and lignin, which are used for synthesis of biopolymers. Polymer-coated noble metal nanoparticles are used in nanobiomedicine and fundamental biomaterials. Section B describes applications of biopolymers in biomedical, antimicrobial, industrial, nanotechnology, laser-based thin films, and regenerative medicines. Section C is dedicated for advancement and engineering in biopolymers for personal protective garments, equipments, membrane separation processes, purifications, and new generation of high-performance biomaterials. A new numerical-cum-graphical method called TI2BioP (Topological Indices to BioPolymers) has been developed to estimate topological indices (TIs) from two-dimensional (2D) graphical approaches for the natural biopolymers DNA, RNA, and proteins.