Cantitate/Preț
Produs
Total produse
Vezi coșul
Prime Editing de Peter M J Quinn

Prime Editing

Editat de Peter M J Quinn, Bruna Lopes Da Costa
en Limba Engleză Hardback – 16 noi 2026

This book looks at the latest advancements in prime editing. The chapters in this book cover pegRNA design and assembly; optimizations for prime editing in mammals; plants and yeast; approaches for quantification of on-target and off-target events; and viral delivery. Written in the highly successfulMethods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and practical, Prime Editing: Methods and Protocols is a valuable resource that provides researchers with detailed and practical methodology for implementing prime editing across a wide range of biological systems.

Citește tot Restrânge

Preț: 117263 lei

Preț vechi: 152290 lei
-23% Precomandă

Puncte Express: 1759

Carte nepublicată încă

Livrare prin curier în România Precomanda se expediază când titlul devine disponibil.
Transport gratuit pentru acest produs Plată online sau ramburs, în funcție de opțiunile comenzii.
Retur gratuit în 14 zile Comandă securizată și suport în română.
 Precomandă

Wish list
Gift list
Am citit!
Adaugă în listă
Doresc să fiu notificat când acest titlu va fi disponibil:
Trimite

Specificații

ISBN-13: 9781071656662
ISBN-10: 107165666X
Pagini: 445
Editura: Springer Us

Cuprins

Prime Editing: Mechanism and Developments.- pegRNA Online Tool Design and Experimental Assembly: Exploring the Practical Use of PE-Designer for Prime Editing.- Design of Highly Efficient pegRNAs with Minimized Off-Target Effects Using DeepPrime.- Using Prime Editing Guide Generator (PEGG) for High-Throughput Generation of Prime Editing Sensor Libraries.- Rapid Cell-Free Preparation of Circular Vector DNA for pegRNA Expression in Prime Editing.- Construction of the p2PE3 Platform Based on RNA-Pol-II-Driven Engineered pegRNA.- Protocol for Prime Editing with Engineered PAM-Flexible Cas9 Variants.- Improvement of Genome Editing Efficiency in Mammalian Cells by A Refined Uni-Vector Prime Editing System.- Optimization and Evaluation of Prime Editing Activity in Human iPSCs.- All-in-One Prime Editing System for Efficient Generation and Isolation of Single Edited hiPSCs Clones.- Large Insertion Using Template-Jumping Prime Editing.- PE-STOP for Precise Installation/Reversion of Nonsense Mutation.- Quantification of Prime Editor-Corrected Frameshift Mutations Using Bioluminescence Resonance Energy Transfer (BRET).- Functional Analysis of Genetic Variants Using High-Throughput Prime Editing Screening.- Digital PCR Workflow for Prime Editing Quantification and Analysis.- TAPE-seq: A Cell-Based Method for Genome-Wide Off-Target Prediction in Prime Editing.- Protocol for Prime Editing in Human Erythroid Cell Lines Using Lentiviral Delivery.- Plant Prime Editing: Design, Assembly, and Application.- Prime Editing-Mediated Genome Modification in Saccharomyces cerevisiae.