This book looks at the latest advancements in prime editing. The chapters in this book cover pegRNA design and assembly; optimizations for prime editing in mammals; plants and yeast; approaches for quantification of on-target and off-target events; and viral delivery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and practical, Prime Editing: Methods and Protocols is a valuable resource that provides researchers with detailed and practical methodology for implementing prime editing across a wide range of biological systems.