This second edition provides new and updated methods focusing on pollen cryopreservation in various crops. Chapters detail specific plant groups, addressing a wide range of species—from cereals and grasses to fruits, nuts, vegetables, ornamentals, and medicinal plants. It features protocols for species such as Semecarpus anacardium, grapes, strawberries, cashew, soybean, amaryllis, and many others, offering practical guidance for researchers and breeders working across diverse agricultural and horticultural domains.Step-by-step protocols are complemented by personal notes, precautions, and specifying the reagents to be used in each step to ensure the repeatability of the procedure across labs. Authoritative and cutting-edge, Pollen Cryopreservation Protocols, Second Edition aims to serve as reference for researchers studying plant breeding and plant conservation biology.