Chromatography is a technique that is used for the separation of a mixture. The solutes or components of a mixture are separated based on the relative proportion of individual solutes distributed between a moving fluid stream and a contiguous stationary phase. Chromatography can be analytical or preparative. Depending on the mode of operation, geometry of the system, phases involved and the retention mechanism, chromatography can be of two types- column chromatography and planar chromatography. These techniques are of immense use in various chemical and biological fields. This book outlines the processes and applications of chromatography in detail. It includes some of the vital pieces of work being conducted across the world, on various topics related to chromatography. It will prove to be immensely beneficial to students and researchers in this field.