Methods of Protein Microcharacterization: A Practical Handbook: Biological Methods
Autor John E. Shivelyen Limba Engleză Hardback – 8 iul 1986
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Specificații
ISBN-13: 9780896030909
ISBN-10: 0896030903
Pagini: 456
Ilustrații: XIX, 456 p.
Dimensiuni: 155 x 235 x 30 mm
Greutate: 0.87 kg
Ediția:1986
Editura: Humana Press Inc.
Colecția Humana
Seria Biological Methods
Locul publicării:Totowa, NJ, United States
ISBN-10: 0896030903
Pagini: 456
Ilustrații: XIX, 456 p.
Dimensiuni: 155 x 235 x 30 mm
Greutate: 0.87 kg
Ediția:1986
Editura: Humana Press Inc.
Colecția Humana
Seria Biological Methods
Locul publicării:Totowa, NJ, United States
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Public țintă
ResearchCuprins
Microisolation Techniques.- Microisolation of Biologically Active Polypeptides by Reverse-Phase Liquid Chromatography.- Reverse-Phase HPLC Isolation and Microsequence Analysis.- Purification of Microgram Quantities of Proteins by Polyacrylamide Gel Electrophoresis.- Microamino Acid Analysis.- Amino Acid Analysis With Postcolumn Fluorescent Derivatization.- Amino Acid Analysis by o-Phthaldialdehyde Precolumn Derivatization and Reverse-Phase HPLC.- Amino Terminal Analysis.- Manual Edman Sequencing System.- Comparison of the Spinning Cup and Gas-Phase Instruments.- Gas-Phase Protein/Peptide Sequencer.- Design of a Multipurpose Sequencer.- Solid-Phase Methods in Protein Microsequence Analysis.- Analysis of Phenylthiohydantoin Amino Acids by HPLC.- Purification and Preparation of Chemicals for Microsequencing With the Edman Degradation.- Carboxyl-Terminal Analysis.- Microsequence Analysis by Enzymatic Methods.- COOH-Terminal Analysis of Polypeptides by Chemical and Chromatographic Methods.-Mass Spectrometric Analysis of Polypeptides.- Polypeptide Sequence Analysis Using Gas Chromatography-Mass Spectrometry.- Fast Atom Bombardment and Secondary Ion Mass Spectrometry of Peptides and Proteins.