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Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Methods in Molecular Biology, cartea 1085

Editat de Yinduo Ji
en Limba Engleză Paperback – 23 aug 2016
Presenting the most up-to-date techniques for the detection, genotyping, and investigation of methicillin-resistant S. aureus (MRSA), this second edition of Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols collects chapters that utilize the power of complete genomic sequences and advanced high-throughput technologies that have pushed this field to its present state.  These enable the development of specific and rapid diagnosis methods, the investigation and elucidation of mechanisms of bacterial evolution to antibiotic resistance and pathogenicity, and the identification of novel targets to develop more potent therapeutic and/or preventive agents.  Written for the Methods in Molecular Biology series, numerous chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and fully updated, Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols, Second Edition serves as a key reference for researchers attempting to set up a new method to study MRSA or even for technicians and scientists working on other pathogens.
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Specificații

ISBN-13: 9781493960569
ISBN-10: 1493960563
Pagini: 363
Ilustrații: X, 353 p. 54 illus., 24 illus. in color.
Dimensiuni: 178 x 254 x 19 mm
Greutate: 0.63 kg
Ediția:Softcover reprint of the original 2nd ed. 2014
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Cuprins

Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus Infections.- Community-Associated Methicillin Resistant Staphylococcus aureus Case Studies.- Rapid Methods for Detection of MRSA in Clinical Specimens.- Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin Resistant Staphylococcus aureus (MRSA).- Internal Transcribed Spacer (ITS)-PCR Identification of MRSA.- Pulsed-Field Gel Electrophoresis Typing of Staphylococcus aureus Isolates.- Multilocus Sequence Typing (MLST) of Staphylococcus aureus.- Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA.- Genetic Interruption of Target Genes for Investigation of Virulence Factors.- Molecular Analysis of Staphylococcal Superantigens.- Investigation of Staphylococcus aureus Adhesion and Invasion of Host Cells.- Investigation of Biofilm Formation in Clinical Isolates of Staphylococcus aureus.- Transcriptomic Analysis of Staphylococcus aureus Using Microarray and Advanced Next-Generation RNA-seq Technologies.- Proteomic Approach to Investigate Pathogenicity and Metabolism of Methicillin-Resistant Staphylococcus aureus.- Metobolomic Investigation of Methicillin Resistant Staphylococcus aureus.- Treatment of Infections Due to Resistant Staphylococcus aureus.- Anti-Infective Drug Development for MRSA.- Animal Models in Drug Development for MRSA.

Textul de pe ultima copertă

Presenting the most up-to-date techniques for the detection, genotyping, and investigation of methicillin-resistant S. aureus (MRSA), this second edition of Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols collects chapters that utilize the power of complete genomic sequences and advanced high-throughput technologies that have pushed this field to its present state.  These enable the development of specific and rapid diagnosis methods, the investigation and elucidation of mechanisms of bacterial evolution to antibiotic resistance and pathogenicity, and the identification of novel targets to develop more potent therapeutic and/or preventive agents.  Written for the Methods in Molecular Biology series, numerous chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and fully updated, Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols, Second Edition serves as a key reference for researchers attempting to set up a new method to study MRSA or even for technicians and scientists working on other pathogens.

Caracteristici

Serves as a valuable starting point for scientists setting up a new method to study MRSA Provides step-by-step detail essential for reproducible results Contains key notes and detailed implementation advice from the experts Includes supplementary material: sn.pub/extras

Recenzii

From the reviews:
"This book … has a unique format focussing on detailed experimental protocols. This is particularly useful if you are trying a technique for the first time, troubleshooting a method, or wishing to understand how a technique works in order to critique its use in the literature. … there is much to recommend in this book, particularly those who are new to the field and want an introduction to techniques in both basic biology and clinical aspects of MRSA." (Jodi Lindsay, Microbiology Today, May, 2008)
"The book is devoted to the multiple laboratory techniques available for technicians and basic scientists. … a good field manual, and clinicians will have an easy to read book on these techniques. … describes well the laboratory techniques available for the study of MRSA. … Most chapters have illustrations in black and white, which are helpful in the overall discussion, and all chapters have a bibliographic list. … it is a good starting point for clinicians and novice scientists in the world of MRSA infections." (Carlos Ernesto Figueroa Castro, Doody’s Review Service, May, 2008)
"A unique compilation of 17 chapters aimed chiefly at the scientific investigation of MRSA … . This book may also serve as a classic methods reference for those geared towards studying MRSA … . In summary, this book represents an excellent reference manual of protocols used to study MRSA, with concise, clear, and well-written introductory sections that can be useful for laboratory investigators and clinicians wishing to get a better understanding of the theory behind scientific methods. … is timely in its arrival." (George Sakoulas, Clinical Infectious Diseases, Vol. 46, May, 2008)