Membrane Biogenesis: Methods and Protocols: Methods in Molecular Biology, cartea 1033
Editat de Doron Rapaport, Johannes M. Herrmannen Limba Engleză Paperback – 23 aug 2016
Extensive and easily applicable, Membrane Biogenesis: Methods and Protocols provides readers with a comprehensive but still concise collection including both basic protocols of rather general application and more specialized methods for specific and novel techniques.
Toate formatele și edițiile | Preț | Express |
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Paperback (1) | 1002.47 lei 38-45 zile | |
Humana Press Inc. – 23 aug 2016 | 1002.47 lei 38-45 zile | |
Hardback (1) | 548.11 lei 38-45 zile | |
Humana Press Inc. – sep 2013 | 548.11 lei 38-45 zile |
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Specificații
ISBN-13: 9781493963041
ISBN-10: 149396304X
Pagini: 418
Ilustrații: XIV, 404 p. 80 illus., 48 illus. in color.
Dimensiuni: 178 x 254 x 22 mm
Greutate: 7.84 kg
Ediția:Softcover reprint of the original 1st ed. 2013
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:Totowa, NJ, United States
ISBN-10: 149396304X
Pagini: 418
Ilustrații: XIV, 404 p. 80 illus., 48 illus. in color.
Dimensiuni: 178 x 254 x 22 mm
Greutate: 7.84 kg
Ediția:Softcover reprint of the original 1st ed. 2013
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:Totowa, NJ, United States
Cuprins
Part I: Investigation of Membrane Lipids
1. Quantitative Analysis of Cellular Lipids by Nano-Electrospray Ionization Mass Spectrometry
Cagakan Özbalci, Timo Sachsenheimer, and Britta Brügger
2. Thin-Layer Chromatography of Phospholipids
Rania M. Deranieh, Amit S. Joshi, and Miriam L. Greenberg
3. Analysis of Membrane Lipid Biogenesis Pathways Using Yeast Genetics
Martina Gsell and Günther Daum
Part II: Analysis of Structure and Topology of Membrane Proteins
4. Using 2D Crystals to Analyze the Structure of Membrane Proteins
Ian Collinson, Janet Vonck, and Dilem Hizlan
5. Crystallization of Membrane Proteins
Florian G. Müller and C. Roy D. Lancaster
6. Molecular Dynamics Simulations of Membrane Proteins
Kristyna Pluhackova, Tsjerk Wassenaar, and Rainer A. Böckmann
7. Site-Specific Fluorescent Probe Labeling of Mitochondrial Membrane Proteins
Christine T. Schwall and Nathan N. Alder
8. Topology Determination of Untagged Membrane Proteins
Iris Nasie, Sonia Steiner-Mordoch, and Shimon Schuldiner
9. self–assembling GFP: A Versatile Tool for Plant (Membrane) Protein Analyses
Katharina Wiesemann, Lucia E. Groß, Manuel Sommer, Enrico Schleiff, and Maik S. Sommer
Part III: Studying Protein-Protein and Protein-Lipids Interactions within Membranes
10. The Use of Cardiolipin-Containing Liposomes as a Model System to Study the Interaction Between Proteins and the Inner Mitochondrial Membrane
Milit Marom and Abdussalam Azem
11. Analysis of the Interaction Between Membrane Proteins and Soluble Binding Partners by Surface Plasmon Resonance
Zht Cheng Wu, Jeanine de Keyzer, Ilja Kusters, and Arnold J.M Driessen
12. Peptide Interaction with and Insertion into Membranes
Ron Saar-Dover, Avraham Ashkenazi, and Yechiel Shai
13. Scanning Fluorescence Correlation Spectroscopy in Model Membrane Systems
Joseph D. Unsay and Ana J. García-Sáez
14. Analyses of Protein-Protein Interactions by In Vivo Photocrosslinking in Budding Yeast
Takuya Shiota, Shuh-ichi Nishikawa, and Toshiya Endo
15. Sedimentation Velocity Analytical Ultracentrifugation in Hydrogenated and Deuterated Solvents for the Characterization of Membrane Proteins
Aline Le Roy, Hugues Nury, Benjamin Wiseman, Jonathan Sarwan, Jean-Michel Jault, and Christine Ebel
16. Membrane Partitioning and Translocation Studied by Isothermal Titration Calorimetry
Carolyn Vargas, Johannes Klingler, and Sandro Keller
Part IV: Membrane Biogenesis and Dynamics
17. Analyzing Membrane Dynamics with Live Cell Fluorescence Microscopy with a Focus on Yeast Mitochondria
Dirk Scholz, Johannes Förtsch, Stefan Böckler, Till Klecker, and Benedikt Westermann
18. Analysis of Protein Translocation into the Endoplasmic Reticulum of Human Cells
Johanna Dudek, Sven Lang, Stefan Schorr, Johannes Linxweiler, Markus Greiner, and Richard Zimmermann
19. An Assay to Monitor the Membrane Integration of Single-Span Proteins
Katrin Krumpe and Doron Rapaport
20. Methods to Study the Biogenesis of Membrane Proteins in Yeast Mitochondria
Daniel Weckbecker and Johannes M. Herrmann
Part V: Functional Analysis of Membrane Proteins
21. Reconstitution of Mitochondrial Presequence Translocase into Proteoliposomes
Martin van der Laan, Ralf M. Zerbes, and Chris van der Does
22. Single Channel Analysis of Membrane Proteins in Artificial Bilayer Membranes
Philipp Bartsch, Anke Harsman, and Richard Wagner
23. Quantification of Protein Complexes by Blue Native Electrophoresis
Juliana Heidler, Valentina Strecker, Florian Csintalan, Lea Bleier, and Ilka Wittig
24. Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) and GFP-Fusions
Anna Hjelm, Susan Schlegel, Thomas Baumgarten, Mirjam Klepsch, David Wickström, David Drew, and Jan-Willem de Gier
1. Quantitative Analysis of Cellular Lipids by Nano-Electrospray Ionization Mass Spectrometry
Cagakan Özbalci, Timo Sachsenheimer, and Britta Brügger
2. Thin-Layer Chromatography of Phospholipids
Rania M. Deranieh, Amit S. Joshi, and Miriam L. Greenberg
3. Analysis of Membrane Lipid Biogenesis Pathways Using Yeast Genetics
Martina Gsell and Günther Daum
Part II: Analysis of Structure and Topology of Membrane Proteins
4. Using 2D Crystals to Analyze the Structure of Membrane Proteins
Ian Collinson, Janet Vonck, and Dilem Hizlan
5. Crystallization of Membrane Proteins
Florian G. Müller and C. Roy D. Lancaster
6. Molecular Dynamics Simulations of Membrane Proteins
Kristyna Pluhackova, Tsjerk Wassenaar, and Rainer A. Böckmann
7. Site-Specific Fluorescent Probe Labeling of Mitochondrial Membrane Proteins
Christine T. Schwall and Nathan N. Alder
8. Topology Determination of Untagged Membrane Proteins
Iris Nasie, Sonia Steiner-Mordoch, and Shimon Schuldiner
9. self–assembling GFP: A Versatile Tool for Plant (Membrane) Protein Analyses
Katharina Wiesemann, Lucia E. Groß, Manuel Sommer, Enrico Schleiff, and Maik S. Sommer
Part III: Studying Protein-Protein and Protein-Lipids Interactions within Membranes
10. The Use of Cardiolipin-Containing Liposomes as a Model System to Study the Interaction Between Proteins and the Inner Mitochondrial Membrane
Milit Marom and Abdussalam Azem
11. Analysis of the Interaction Between Membrane Proteins and Soluble Binding Partners by Surface Plasmon Resonance
Zht Cheng Wu, Jeanine de Keyzer, Ilja Kusters, and Arnold J.M Driessen
12. Peptide Interaction with and Insertion into Membranes
Ron Saar-Dover, Avraham Ashkenazi, and Yechiel Shai
13. Scanning Fluorescence Correlation Spectroscopy in Model Membrane Systems
Joseph D. Unsay and Ana J. García-Sáez
14. Analyses of Protein-Protein Interactions by In Vivo Photocrosslinking in Budding Yeast
Takuya Shiota, Shuh-ichi Nishikawa, and Toshiya Endo
15. Sedimentation Velocity Analytical Ultracentrifugation in Hydrogenated and Deuterated Solvents for the Characterization of Membrane Proteins
Aline Le Roy, Hugues Nury, Benjamin Wiseman, Jonathan Sarwan, Jean-Michel Jault, and Christine Ebel
16. Membrane Partitioning and Translocation Studied by Isothermal Titration Calorimetry
Carolyn Vargas, Johannes Klingler, and Sandro Keller
Part IV: Membrane Biogenesis and Dynamics
17. Analyzing Membrane Dynamics with Live Cell Fluorescence Microscopy with a Focus on Yeast Mitochondria
Dirk Scholz, Johannes Förtsch, Stefan Böckler, Till Klecker, and Benedikt Westermann
18. Analysis of Protein Translocation into the Endoplasmic Reticulum of Human Cells
Johanna Dudek, Sven Lang, Stefan Schorr, Johannes Linxweiler, Markus Greiner, and Richard Zimmermann
19. An Assay to Monitor the Membrane Integration of Single-Span Proteins
Katrin Krumpe and Doron Rapaport
20. Methods to Study the Biogenesis of Membrane Proteins in Yeast Mitochondria
Daniel Weckbecker and Johannes M. Herrmann
Part V: Functional Analysis of Membrane Proteins
21. Reconstitution of Mitochondrial Presequence Translocase into Proteoliposomes
Martin van der Laan, Ralf M. Zerbes, and Chris van der Does
22. Single Channel Analysis of Membrane Proteins in Artificial Bilayer Membranes
Philipp Bartsch, Anke Harsman, and Richard Wagner
23. Quantification of Protein Complexes by Blue Native Electrophoresis
Juliana Heidler, Valentina Strecker, Florian Csintalan, Lea Bleier, and Ilka Wittig
24. Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) and GFP-Fusions
Anna Hjelm, Susan Schlegel, Thomas Baumgarten, Mirjam Klepsch, David Wickström, David Drew, and Jan-Willem de Gier
Textul de pe ultima copertă
Membrane proteins and membrane lipids form complex interactive systems that are highly dynamic and able to be studied only by combinations of different in vivo and in vitro techniques. In Membrane Biogenesis: Methods and Protocols, experts in the field present a broad collection of methods to study the biogenesis and function of cellular membranes. Beginning with how membrane lipids or membrane proteins can be studied, this detailed volume continues with sections covering different procedures to investigate the interaction of membrane proteins among each other or with membrane lipids, methods to study the biogenesis of membrane proteins and the dynamics of organelles, as well as protocols for the analyses of the functions or complex organization of membrane proteins. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Extensive and easily applicable, Membrane Biogenesis: Methods and Protocols provides readers with a comprehensive but still concise collection including both basic protocols of rather general application and more specialized methods for specific and novel techniques.
Extensive and easily applicable, Membrane Biogenesis: Methods and Protocols provides readers with a comprehensive but still concise collection including both basic protocols of rather general application and more specialized methods for specific and novel techniques.
Caracteristici
Features numerous techniques to study membrane proteins and membrane lipids as well as their interactions
Includes detailed, lab-ready protocols and methodologies
Contains expert tips and key implementation advice to ensure successful results
Includes detailed, lab-ready protocols and methodologies
Contains expert tips and key implementation advice to ensure successful results