Human Stem Cell Manual: A Laboratory Guide
Editat de Suzanne Peterson, Jeanne F. Loringen Limba Engleză Hardback – 31 aug 2025
- Offers a comprehensive guide for medical and biology researchers who want to use stem cells for basic research, disease modeling, drug development, and cell therapy applications
- Provides a cohesive global view of the current state of stem cell research, with chapters written by pioneering stem cell researchers in Asia, Europe, and North America
- Includes chapters devoted to recently developed methods, such as iPSC technology, written by the scientists who made these breakthroughs
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Specificații
ISBN-13: 9780128224526
ISBN-10: 0128224525
Pagini: 656
Ilustrații: 150 illustrations (50 in full color)
Dimensiuni: 191 x 235 mm
Ediția:3
Editura: ELSEVIER SCIENCE
ISBN-10: 0128224525
Pagini: 656
Ilustrații: 150 illustrations (50 in full color)
Dimensiuni: 191 x 235 mm
Ediția:3
Editura: ELSEVIER SCIENCE
Cuprins
Part I: Basic methods: Maintenance of stem cell cultures 1. Human Embryonic Stem Cell Culture 2. Feeder layers and feeder-free culture 3. Mouse Embryonic Fibroblast Feeder Cells 4. Cryopreservation of hESC Part II: Basic methods: Characterization of stem cells 5. Classical Cytogenetics: Karyotyping 6. Spectral Karyotyping and Fluorescent in situ Hybridization 7. Using SNP Genotyping to Identify Cell Lines, Determine Genomic Abnormalities and Monitor DNA Methylation Patterns 8. FACS/Cell sorting 9. Fluorescence Immunocytochemical Analysis of Stem Cells 10. Characterization of Stem Cells Using RT-PCR 11. Gene Expression Profiling of Stem Cells by Microarray 12. Teratomas produced from Human Embryonic Stem Cells Xenografted into Immunodeficient Mice 13. Generation of HESC-Derived Teratomas Part III: Differentiation of stem cells 14. “Embryoid Body” Formation and Neuroepithelia Differentiation 15. Motor Neuron and Dopamine Neuron Differentiation 16. Oligodendrocyte Differentiation from hESC 17. Cardiac 18. Hematopoietic Part IV: Genetic manipulation of stem cells 19. Genetic Manipulation of hESC: Lentivirus Vectors 20. Methods developed for mouse ES cells Part V: Advanced methods 21. Derivation of human ES cells 22. Neural Stem Cell Culture 23. Transplantation of stem cells 24. In vitro fertilization 25. Functional characterization-neural cells Part VI: Practical issues in establishing a human ES cell laboratory 26. Setting Up a Facility for hESC Research Part VII: Essays on stem cell biology 27. Intellectual Property: Owning the Stem Cell 28. Ethical Concerns for Stem Cell Research 29. Guidelines for hESC Research Oversight (ESCRO) Committees