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Histology Protocols de Tim D Hewitson

Histology Protocols

Editat de Tim D Hewitson, Ian A Darby
en Limba Engleză Hardback – 16 dec 2009

EDIȚIA: Volumul Histology Protocols, publicat de Humana Press Inc., reprezintă o resursă tehnică esențială care consolidează puntea dintre histologia clasică și biologia moleculară modernă. Remarcăm efortul editorilor Tim D Hewitson și Ian A Darby de a depăși bariera dintre seturile de abilități considerate anterior reciproc exclusive, oferind protocoale care permit biologilor moleculari să utilizeze resursele histologice la potențial maxim. Structura cărții este riguros segmentată pentru a facilita fluxul de lucru în laborator. Prima parte se concentrează pe procesarea țesuturilor, incluzând capitole critice despre fixare și recuperarea antigenului, dar și metode optimizate pentru extracția ARN-ului din probe de arhivă. Reținem progresia logică spre Partea a II-a, unde sunt detaliate tehnici de colorare de specialitate, de la imunohistochimia dublă la histochimia lectinelor și hibridizarea in situ. Ultima secțiune subliniază importanța analizei de imagine și a noilor tehnologii de vizualizare. Această abordare metodologică extinde cadrul propus de Manual of Histological Techniques de Santosh Kumar Mondal prin accentul pus pe mecanismele moleculare și datele noi privind microdisecția prin captură laser. Poziționarea lucrării în contextul operei editorilor este evidentă; experiența acumulată în Kidney Research se traduce aici într-o precizie chirurgicală a protocoalelor, în special în analiza expresiei genetice la nivelul glomerulului renal. Spre deosebire de manualele generale, acest volum din seria „Methods in Molecular Biology” se concentrează pe reproductibilitatea pașilor de laborator, fiind un instrument de lucru indispensabil pentru cercetarea patogenezei bolilor umane.

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Specificații

ISBN-13: 9781603273442
ISBN-10: 1603273441
Pagini: 229
Ilustrații: X, 229 p.
Dimensiuni: 178 x 254 x 14 mm
Greutate: 0.64 kg
Ediția:2010 edition
Editura: Humana Press Inc.
Locul publicării:Totowa, NJ, United States

Public țintă

Professional/practitioner

De ce să citești această carte

Subliniem utilitatea acestei cărți pentru cercetătorii și practicienii care doresc să combine rigoarea histopatologiei cu precizia biologiei moleculare. Cititorul câștigă acces la protocoale verificate pentru manipularea țesuturilor arhivate și tehnici avansate de marcare celulară (apoptoză, hipoxie). Este o investiție strategică pentru orice laborator de patologie care urmărește implementarea metodelor de ultimă oră în analiza moleculară a probelor tisulare.

Descriere scurtă

Somuchofwhatweknowaboutthepathogenesisofhumandiseasehascomefromthe systematic and careful study of histological material. Indeed, every internal medicine discipline has its landmark papers describing the clinico-pathological correlations. However, increasingly, it is molecular and cellular biology that provides the necessary mechanistic insights. For many years, it was thought that the two skill sets were mutually exclusive, but we hope that this book shows that this is not necessarily so. Implicitinthescienceofhistologyisthepreservationandarchivingoftissue.PartIof the book concentrates on the preparation of tissue, providing an overview of fixation, embedding, and processing (Chapter 1), and in Chapters 2 and 3, the required techniques for the retrieval of RNA from histological sections. Both routine and specialist histological staining techniques are provided in Part II. These include pro- cols for immuno (Chapters 4–7), lectin (Chapter 8), and hybridization (Chapter 9) histochemistry,histologicalstaining (Chapters10and11),aswellasspecificmethods for the in situ identification of hypoxia (Chapter 12) and apoptosis (Chapter 13). Finally, Part III details advances in imaging (Chapters 14–16) and image analysis (Chapter 17). It is hoped that this volume will provide molecular biologists with the basic his- chemical techniques and histologists with the molecular techniques to realise the potential of their resource. We are indebted to the authors for their generosity in sharing these protocols.

Cuprins

Tissue Processing.- Tissue Preparation for Histochemistry: Fixation, Embedding, and Antigen Retrieval for Light Microscopy.- An Optimized RNA Extraction Method from Archival Formalin-Fixed Paraffin-Embedded Tissue.- Laser-Capture Microdissection and Pressure Catapulting for the Analysis of Gene Expression in the Renal Glomerulus.- Staining Techniques.- Immunofluorescence Detection of the Cytoskeleton and Extracellular Matrix in Tissue and Cultured Cells.- Double Immunohistochemistry with Horseradish Peroxidase and Alkaline Phosphatase Detection Systems.- Retrogradely Transported Neuronal Tracers Combined with Immunohistochemistry Using Free-Floating Brain Sections.- High-Pressure Freezing, Chemical Fixation and Freeze-Substitution for Immuno-electron Microscopy.- Lectin Histochemistry for Light and Electron Microscopy.- Duplex In Situ Hybridization in the Study of Gene Co-regulation in the Vertebrate Brain.- Special Stains for Extracellular Matrix.- Active Staining of Mouse Embryos for Magnetic Resonance Microscopy.- Immunohistochemical Detection of Tumour Hypoxia.- In Situ Localization of Apoptosis Using TUNEL.- Imaging Techniques.- Use of Confocal Microscopy for Three-Dimensional Imaging of Neurons in the Spinal Cord.- High-Resolution Confocal Imaging in Tissue.- Software-Based Stacking Techniques to Enhance Depth of Field and Dynamic Range in Digital Photomicrography.- Image Analysis and Quantitative Morphology.

Recenzii

From the reviews:
“This slim volume aims to provide molecular biologists with the basic histochemical techniques and histologists with the molecular techniques to realise their potential of their resource. … I enjoyed reading this book … . this book is not particularly expensive and is so comprehensive and well written that I would certainly recommend it to anyone thinking of setting up a research project but uncertain what sort of method to use. It would also be a valuable asset to any departmental library … .” (Anne Patricia Campbell, Bulletin of the Royal Society of Pathologists, Issue 154, April, 2011)
“Reports the basic techniques of immunohistochemistry and molecular biology used to optimize resources and obtain accurate results. … This book is recommended to students and researchers using the laboratory techniques on histology described. Updated protocols are described by renowned scientists in the area using understandable language while details of possible interference are given for each technique. Tim D. Hewiston and Ian A. Darby have undertaken several studies on histology, many of which were conducted in collaboration with the authors contributing to this book.” (Rui Curi, Brazilian Journal of Pharmaceutical Sciences, Spring, 2011)
“This volume in the Methods in Molecular Biology series provides a compendium of proven protocols for modern histology. … Written for basic science and clinical researchers interested in mapping the location and distribution of biomarkers, the book also will be appreciated by lecturers and students specializing in biomedical technology, histology, and clinical laboratory medicine. … The book is well written and carefully edited, and will be a welcome resource for all basic science and clinical researchers studying modern cell biology.” (Bruce A. Fenderson, Doody’s Review Service, June, 2010)

Textul de pe ultima copertă

With much of what we know about the pathogenesis of disease coming from the systematic and careful study of histological material, histology has proven to be an incredibly valuable area of study. In Histology Protocols, expert researchers in the field contribute detailed methods involving the preparation of tissue, providing an overview of fixation, embedding, and processing, along with the required techniques for the retrieval of RNA from histological sections, followed by sections on routine and specialist histological staining techniques, including immuno-, lectin, and hybridization histochemistry, histological staining, as well as specific methods for the in situ identification of hypoxia and apoptosis, and details on the advances in imaging and image analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Histology Protocols will provide molecular biologists with the basic histochemical techniques and histologists with the molecular techniques necessary to realize the potential of their resource.

Caracteristici

Provides an easily accessible reference volume for histological techniques written by leading researchers in the field Details the techniques for investigating the expression of many different types of targets in a variety of tissue and samples from sections and cells to electron microscopy Covers a wide range of techniques from classical histochemistry to detection of mRNA, apoptosis, and hypoxia Step-by-step guide to techniques including extensive notes to aid in troubleshooting techniques Includes supplementary material: sn.pub/extras