The technique used in analytical chemistry to identify, separate and quantify each element in a mixture is known as high performance liquid chromatography. It was initially known as high pressure liquid chromatography. For the separation of the component, it relies on the process of pumping to pass the liquid through the adsorbent. The various parameters in this field are particle size, pump pressure, internal diameter, pore size, autosamplers and detectors. Partition chromatography, displacement chromatography, normal-phase chromatography, size exclusion chromatography, bioaffinity chromatography and aqueous normal phase chromatography are some of its types. The field has applications in manufacturing, detecting illicit drugs, research and medicine. This book is a valuable compilation of topics, ranging from basic to the most complex advancements in high performance liquid chromatography. The various studies that are constantly contributing towards the advancing technologies and evolution of this field are examined in detail. For all those readers who are interested in this area of study, the case studies included in this book will serve as an excellent guide to develop a comprehensive understanding.