Fluorescent Dyes
Autor Jiangli Fan, Xiaojun Pengen Limba Engleză Hardback – 24 feb 2027
Fluorescent dye design, synthesis, and biomedical application in one reference
Developing fluorescent dyes with the specificity, sensitivity, and photostability required for biological systems demands integrated knowledge spanning organic synthesis, photophysics, and biomedicine. Fluorescent Dyes: Synthesis and Biomedical Applications consolidates decades of progress into a single reference, covering fluorescence emission principles, rational dye design, and translational applications in sensing, labeling, bioimaging, medical diagnosis, and cancer treatment.
The book details fluorescent chemosensor design for selective recognition of bioactive small molecules in living systems, fluorescence-labeling agents targeting proteins and subcellular organelles, dyes engineered for high-resolution imaging, and theranostic agents combining diagnosis with therapy. Each topic addresses structure-property relationships governing performance under rigorous biological conditions, alongside future development directions for the field.
Readers will also find:
- New approaches for rational design of high-performance fluorescent dyes meeting the demanding requirements of in vivo biological applications
- Coverage of photodynamic therapy photosensitizers and their role in targeted cancer treatment strategies using fluorescence-guided approaches
- Analysis of fluorescence phenomena from fundamental photophysical principles through to practical probe construction and deployment in cellular environments
- Discussion of targeted nanodrug delivery systems incorporating fluorescent dyes for combined imaging and therapeutic functionality in living systems
- Evaluation of representative fluorescent dye classes developed over recent decades with detailed synthesis routes and characterization data
Designed for photochemists, cell biologists, pharmaceutical chemists, biochemists, organic chemists, analytical chemists, and sensor industry professionals, this reference provides the design principles, synthetic methods, and application frameworks needed to advance fluorescent dye research from molecular design through clinical translation.
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Specificații
ISBN-13: 9783527353460
ISBN-10: 3527353461
Pagini: 416
Ilustrații: 103 farbige Abbildungen
Dimensiuni: 170 x 244 mm
Editura: Wiley-VCH GmbH
Locul publicării:Weinheim, Germany
ISBN-10: 3527353461
Pagini: 416
Ilustrații: 103 farbige Abbildungen
Dimensiuni: 170 x 244 mm
Editura: Wiley-VCH GmbH
Locul publicării:Weinheim, Germany
Notă biografică
Jiangli Fan, PhD, is Professor at the State Key Laboratory of Fine Chemicals, Dalian University of Technology, China. Her research focuses on small-molecule fluorescent probes for bioimaging and sensing, photosensitizer-based photodynamic therapy, and targeted nanodrug delivery. She serves on the editorial boards of Dyes and Pigments and Scientific Reports, and is a recipient of China's national outstanding youth fund.
Xiaojun Peng, PhD, is Director of the State Key Laboratory of Fine Chemicals at Dalian University of Technology, China, and editor-in-chief of Smart Molecules. Elected a member of the Chinese Academy of Sciences in 2017, his research covers dyes for fluorescent bioimaging, labeling, and digital printing. He has published more than 300 research papers with approximately 16,500 citations.
Cuprins
Chapter 1: Introduction
1.1. Development of fluorescence and phosphorescence
1.2. Mechanism of fluorescence and phosphorescence
Chapter 2: The Rational Design of High-Performance Near Infrared Fluorescent Dyes
2.1. Introduction
2.2. Design rationale of NIR absorbing and emitting dyes
2.2.1. The necessity of a D-p-A scaffold
2.2.2. Further modulation of the HOMO-LUMO gap of a D-p-A scaffold
2.2.3. Rigidification of D-p-A scaffold
2.2.4. Steric protection of the D-p-A scaffold
2.3. Conclusion and outlook
Chapter 3: Fluorescent Dyes for Organelle Staining
3.1. Overview of organelle-staining dyes
3.2. Fluorescent dye for cell membrane staining
3.2.1. Biomedical functions of the cell membrane
3.2.2. Cell membrane staining fluorescent dyes and their applications
3.3. Fluorescent dye for mitochondrial staining
3.3.1. Biomedical functions of mitochondria
3.3.2. Mitochondrial staining fluorescent dyes and their applications
3.4 Fluorescent dye for lysosome staining
3.4.1. Biomedical functions of lysosomes
3.4.2. Lysosome staining fluorescent dyes and their applications
3.5 Fluorescent dye for Golgi apparatus staining
3.5.1. Biomedical functions of the Golgi apparatus
3.5.2. Golgi staining fluorescent dyes and their applications
3.6. Fluorescent dye for endoplasmic reticulum staining
3.6.1. Biomedical functions of the endoplasmic reticulum
3.6.2. Fluorescent dyes for endoplasmic reticulum staining and their applications
3.7. Fluorescent dye for cell nucleus staining
3.7.1. Biomedical functions of the nucleus
3.7.2. Nuclear staining fluorescent dyes and their applications
Chapter 4: Environment-Sensitive Fluorescent Dyes
4.1. Polarity-sensitive fluorescent dyes
4.1.1. Naphthalimide-based polarity-sensitive fluorescent dyes
4.1.2. Coumarin-based polarity-sensitive fluorescent dyes
4.1.3. Nile red-based polarity-sensitive fluorescent dyes
4.1.4. Naphthalene-based polarity-sensitive fluorescent dyes
4.2. Temperature-sensitive fluorescent dyes
4.2.1. Organic small molecule-based temperature-sensitive fluorescent dyes
4.2.2. Polymeric macromolecule-based temperature-sensitive fluorescent dyes
4.2.3. Nanomaterial-based temperature-sensitive fluorescent dyes
4.3. Viscosity-sensitive fluorescent dyes
4.3.1. BODIPY-based viscosity-sensitive fluorescent dyes
4.3.2. DCVJ-based viscosity-sensitive fluorescent dyes
4.3.3. Cyanine-based viscosity-sensitive fluorescent dyes
4.4. pH-sensitive fluorescent dyes
4.4.1. pH-sensitive fluorescent dyes based on reversible protonation of amines
4.4.2. pH-sensitive fluorescent dyes based on protonation of N-heterocycles
4.4.3. pH-sensitive fluorescent dyes based on phenols
4.4.4. pH-sensitive fluorescent dyes based on ring-opening of rhodamine
4.5. Prospects of environment-sensitive fluorescent dyes in biomedical applications
Chapter 5: Fluorescent Dyes for Intracellular Imaging of Ions
5.1. Fluorescent dyes for recognition of alkaline and alkaline earth metal ions
5.1.1. Fluorescent dyes for recognition of calcium ion
5.1.2. Fluorescent dyes for recognition of magnesium ion
5.1.3. Fluorescent dyes for recognition of sodium ion
5.1.4. Fluorescent dyes for recognition of potassium ion
5.2. Fluorescent dyes for recognition of main transition metal ions
5.2.1. Fluorescent dyes for recognition of zinc ion
5.2.2. Fluorescent dyes for recognition of iron and ferrous ions
5.2.3. Fluorescent dyes for recognition of copper ion
5.3. Fluorescent dyes for recognition of anions
5.3.1. Fluorescent dyes for recognition of halide ions
5.3.2. Fluorescent dyes for recognition of phosphate and pyrophosphate
5.4. Fluorescent dyes for recognition of harmful heavy metal ions
5.4.1. Fluorescent dyes for recognition of mercury ion
5.4.2. Fluorescent dyes for recognition of cadmium ion
5.4.3. Fluorescent dyes for recognition of lead ion
5.4.4. Fluorescent dyes for recognition of palladium ion
Chapter 6: Fluorescent Dyes for Recognition of Bioactive Small Molecules
6.1. Fluorescent dyes for recognition of reactive oxygen species
6.1.1. Fluorescent dyes for recognition of singlet oxygen
6.1.2. Fluorescent dyes for recognition of hydrogen peroxide
6.1.3. Fluorescent dyes for recognition of superoxide anion
6.1.4. Fluorescent dyes for recognition of hydroxyl radical
6.1.5. Fluorescent dyes for recognition of hypochlorous acid
6.1.6. Fluorescent dyes for recognition of peroxynitrite
6.2. Fluorescent dyes for recognition of biomercaptan compounds
6.2.1. Fluorescent dyes for recognition of cysteine and homocysteine
6.2.2. Fluorescent dyes for recognition of glutathione
6.3. Fluorescent dyes for recognition of gasotransmitters
6.3.1. Fluorescent dyes for recognition of nitric oxide
6.3.2. Fluorescent dyes for recognition of hydrogen sulfide
6.3.3. Fluorescent dyes for recognition of carbon monoxide
Chapter 7: Fluorescent Probes to Detect Enzymatic Activity
7.1. Introduction
7.2. Fluorescent probes for oxidoreductases
7.2.1. Fluorescent probes for cyclooxygenase
7.2.2. Fluorescent probes for tyrosinase
7.2.3. Fluorescent probes for nitroreductase
7.2.4. Fluorescent probes for monoamine oxidase
7.2.5. Fluorescent probes for peroxidase
7.3. Fluorescent probes for transferases
7.3.1. Fluorescent probes for glutamyl transferase
7.3.2. Fluorescent probes for sulfate transferase
7.3.3. Fluorescent probes for methyl transferase
7.4. Fluorescent probes for hydrolases
7.4.1. Fluorescent probes for proteinase
7.4.2. Fluorescent probes for carboxylesterase
7.4.3. Fluorescent probes for phosphatase
7.5 Conclusion
Chapter 8: Development of Functional Dyes Sensing Nucleic Acid
8.1. The principle of nucleic acid
8.2. The categories of sensing mechanism
8.2.1. Electrostatic interaction
8.2.2. Groove combination
8.2.3. Embedded interaction
8.3. Fluorescent dyes for sensing DNA
8.3.1. Alkaline dyes for DNA
8.3.2. Cationic dyes
8.3.3. Other dyes
8.4. Fluorescent dyes for sensing RNA
8.5. Fluorescent dyes for sensing G-quadruplex
8.5.1. Dyes for DNA G-quadruplex
8.5.2. Dyes for RNA G-quadruplex
8.6. Functional dyes or emissive compounds in living system for advanced imaging
8.6.1. Superresolution probes for nucleus
8.6.2. Superresolution probes for nuclear microenvironment
8.6.3. Emissive analogs of secondary messengers and cofactors
Chapter 9: Development of Fluorescent Probes for Sensing and Labelling Proteins
9.1. Techniques for specifically labeling of proteins
9.2. Labelling strategies base on small molecule ligand
9.2.1. Fluorescent probes for labelling cytoskeleton
9.2.2. Fluorescent probes for labelling ATP-sensitive potassium channel protein
9.3. Labelling strategies base on peptide
9.3.1. a dye-tetracysteine polypeptides and similar labelling dyes
9.3.2. Random screening of dye-peptide specific binding from peptide library
9.4. Labelling strategies base on protein tags
9.4.1. SNAP Tag
9.4.2. Halo Tag
9.5. Labelling strategies base on nucleic acid aptamers
9.6. Conclusion and outlook
Chapter 10: Dyes with Thermally Activated Delayed Fluorescence
10.1. Basic information about TADF dyes
10.1.1. Concepts and luminescence mechanisms of TADF dyes
10.1.2. Physical parameters involved in TADF dyes
10.1.3. Molecular structures and designing principles of TADF dyes
10.2. TADF dyes used as fluorescence bioimaging probes
10.3. TADF dyes used as theranostic photosensitizers
10.4. Perspectives in biological and medical field of TADF dyes.
Chapter 11: Fluorescent Dyes for Super-Resolution Imaging
11.1. Introduction
11.2. Super-resolution imaging techniques
11.3. Fluorescent dyes
11.3.1. Dyes for STED
11.3.2. Dyes for SMLM
11.4. Summary and outlook
Chapter 12: Fluorescent Molecular Prodrug
12.1. Introduction
12.2. Reductive thiol-activated prodrug systems
12.3. Hydrogen peroxide-activated prodrug systems
12.4. Enzyme-activated prodrug systems
12.5. Acidic pH-activated prodrug systems
12.6. External light-activated prodrug systems
Chapter 13: Photosensitive Dyes for Photon-Triggered Cancer Therapy
13.1. Introduction
13.2. Photosensitive dyes for photodynamic therapy
13.2.1. Type II mechanism photosensitizers
13.2.2. Type I mechanism photosensitizers
13.2.3. Clinical photosensitive drugs
13.3. Photosensitive dyes for photothermal therapy
13.3.1. Porphyrins
13.3.2. Cyanines
13.3.3. Phthalocyanines
13.3.4. Diketopyrrolopyrrole dyes
13.3.5. Croconic acid dyes
13.3.6. Bodipys
13.4. Conclusion
1.1. Development of fluorescence and phosphorescence
1.2. Mechanism of fluorescence and phosphorescence
Chapter 2: The Rational Design of High-Performance Near Infrared Fluorescent Dyes
2.1. Introduction
2.2. Design rationale of NIR absorbing and emitting dyes
2.2.1. The necessity of a D-p-A scaffold
2.2.2. Further modulation of the HOMO-LUMO gap of a D-p-A scaffold
2.2.3. Rigidification of D-p-A scaffold
2.2.4. Steric protection of the D-p-A scaffold
2.3. Conclusion and outlook
Chapter 3: Fluorescent Dyes for Organelle Staining
3.1. Overview of organelle-staining dyes
3.2. Fluorescent dye for cell membrane staining
3.2.1. Biomedical functions of the cell membrane
3.2.2. Cell membrane staining fluorescent dyes and their applications
3.3. Fluorescent dye for mitochondrial staining
3.3.1. Biomedical functions of mitochondria
3.3.2. Mitochondrial staining fluorescent dyes and their applications
3.4 Fluorescent dye for lysosome staining
3.4.1. Biomedical functions of lysosomes
3.4.2. Lysosome staining fluorescent dyes and their applications
3.5 Fluorescent dye for Golgi apparatus staining
3.5.1. Biomedical functions of the Golgi apparatus
3.5.2. Golgi staining fluorescent dyes and their applications
3.6. Fluorescent dye for endoplasmic reticulum staining
3.6.1. Biomedical functions of the endoplasmic reticulum
3.6.2. Fluorescent dyes for endoplasmic reticulum staining and their applications
3.7. Fluorescent dye for cell nucleus staining
3.7.1. Biomedical functions of the nucleus
3.7.2. Nuclear staining fluorescent dyes and their applications
Chapter 4: Environment-Sensitive Fluorescent Dyes
4.1. Polarity-sensitive fluorescent dyes
4.1.1. Naphthalimide-based polarity-sensitive fluorescent dyes
4.1.2. Coumarin-based polarity-sensitive fluorescent dyes
4.1.3. Nile red-based polarity-sensitive fluorescent dyes
4.1.4. Naphthalene-based polarity-sensitive fluorescent dyes
4.2. Temperature-sensitive fluorescent dyes
4.2.1. Organic small molecule-based temperature-sensitive fluorescent dyes
4.2.2. Polymeric macromolecule-based temperature-sensitive fluorescent dyes
4.2.3. Nanomaterial-based temperature-sensitive fluorescent dyes
4.3. Viscosity-sensitive fluorescent dyes
4.3.1. BODIPY-based viscosity-sensitive fluorescent dyes
4.3.2. DCVJ-based viscosity-sensitive fluorescent dyes
4.3.3. Cyanine-based viscosity-sensitive fluorescent dyes
4.4. pH-sensitive fluorescent dyes
4.4.1. pH-sensitive fluorescent dyes based on reversible protonation of amines
4.4.2. pH-sensitive fluorescent dyes based on protonation of N-heterocycles
4.4.3. pH-sensitive fluorescent dyes based on phenols
4.4.4. pH-sensitive fluorescent dyes based on ring-opening of rhodamine
4.5. Prospects of environment-sensitive fluorescent dyes in biomedical applications
Chapter 5: Fluorescent Dyes for Intracellular Imaging of Ions
5.1. Fluorescent dyes for recognition of alkaline and alkaline earth metal ions
5.1.1. Fluorescent dyes for recognition of calcium ion
5.1.2. Fluorescent dyes for recognition of magnesium ion
5.1.3. Fluorescent dyes for recognition of sodium ion
5.1.4. Fluorescent dyes for recognition of potassium ion
5.2. Fluorescent dyes for recognition of main transition metal ions
5.2.1. Fluorescent dyes for recognition of zinc ion
5.2.2. Fluorescent dyes for recognition of iron and ferrous ions
5.2.3. Fluorescent dyes for recognition of copper ion
5.3. Fluorescent dyes for recognition of anions
5.3.1. Fluorescent dyes for recognition of halide ions
5.3.2. Fluorescent dyes for recognition of phosphate and pyrophosphate
5.4. Fluorescent dyes for recognition of harmful heavy metal ions
5.4.1. Fluorescent dyes for recognition of mercury ion
5.4.2. Fluorescent dyes for recognition of cadmium ion
5.4.3. Fluorescent dyes for recognition of lead ion
5.4.4. Fluorescent dyes for recognition of palladium ion
Chapter 6: Fluorescent Dyes for Recognition of Bioactive Small Molecules
6.1. Fluorescent dyes for recognition of reactive oxygen species
6.1.1. Fluorescent dyes for recognition of singlet oxygen
6.1.2. Fluorescent dyes for recognition of hydrogen peroxide
6.1.3. Fluorescent dyes for recognition of superoxide anion
6.1.4. Fluorescent dyes for recognition of hydroxyl radical
6.1.5. Fluorescent dyes for recognition of hypochlorous acid
6.1.6. Fluorescent dyes for recognition of peroxynitrite
6.2. Fluorescent dyes for recognition of biomercaptan compounds
6.2.1. Fluorescent dyes for recognition of cysteine and homocysteine
6.2.2. Fluorescent dyes for recognition of glutathione
6.3. Fluorescent dyes for recognition of gasotransmitters
6.3.1. Fluorescent dyes for recognition of nitric oxide
6.3.2. Fluorescent dyes for recognition of hydrogen sulfide
6.3.3. Fluorescent dyes for recognition of carbon monoxide
Chapter 7: Fluorescent Probes to Detect Enzymatic Activity
7.1. Introduction
7.2. Fluorescent probes for oxidoreductases
7.2.1. Fluorescent probes for cyclooxygenase
7.2.2. Fluorescent probes for tyrosinase
7.2.3. Fluorescent probes for nitroreductase
7.2.4. Fluorescent probes for monoamine oxidase
7.2.5. Fluorescent probes for peroxidase
7.3. Fluorescent probes for transferases
7.3.1. Fluorescent probes for glutamyl transferase
7.3.2. Fluorescent probes for sulfate transferase
7.3.3. Fluorescent probes for methyl transferase
7.4. Fluorescent probes for hydrolases
7.4.1. Fluorescent probes for proteinase
7.4.2. Fluorescent probes for carboxylesterase
7.4.3. Fluorescent probes for phosphatase
7.5 Conclusion
Chapter 8: Development of Functional Dyes Sensing Nucleic Acid
8.1. The principle of nucleic acid
8.2. The categories of sensing mechanism
8.2.1. Electrostatic interaction
8.2.2. Groove combination
8.2.3. Embedded interaction
8.3. Fluorescent dyes for sensing DNA
8.3.1. Alkaline dyes for DNA
8.3.2. Cationic dyes
8.3.3. Other dyes
8.4. Fluorescent dyes for sensing RNA
8.5. Fluorescent dyes for sensing G-quadruplex
8.5.1. Dyes for DNA G-quadruplex
8.5.2. Dyes for RNA G-quadruplex
8.6. Functional dyes or emissive compounds in living system for advanced imaging
8.6.1. Superresolution probes for nucleus
8.6.2. Superresolution probes for nuclear microenvironment
8.6.3. Emissive analogs of secondary messengers and cofactors
Chapter 9: Development of Fluorescent Probes for Sensing and Labelling Proteins
9.1. Techniques for specifically labeling of proteins
9.2. Labelling strategies base on small molecule ligand
9.2.1. Fluorescent probes for labelling cytoskeleton
9.2.2. Fluorescent probes for labelling ATP-sensitive potassium channel protein
9.3. Labelling strategies base on peptide
9.3.1. a dye-tetracysteine polypeptides and similar labelling dyes
9.3.2. Random screening of dye-peptide specific binding from peptide library
9.4. Labelling strategies base on protein tags
9.4.1. SNAP Tag
9.4.2. Halo Tag
9.5. Labelling strategies base on nucleic acid aptamers
9.6. Conclusion and outlook
Chapter 10: Dyes with Thermally Activated Delayed Fluorescence
10.1. Basic information about TADF dyes
10.1.1. Concepts and luminescence mechanisms of TADF dyes
10.1.2. Physical parameters involved in TADF dyes
10.1.3. Molecular structures and designing principles of TADF dyes
10.2. TADF dyes used as fluorescence bioimaging probes
10.3. TADF dyes used as theranostic photosensitizers
10.4. Perspectives in biological and medical field of TADF dyes.
Chapter 11: Fluorescent Dyes for Super-Resolution Imaging
11.1. Introduction
11.2. Super-resolution imaging techniques
11.3. Fluorescent dyes
11.3.1. Dyes for STED
11.3.2. Dyes for SMLM
11.4. Summary and outlook
Chapter 12: Fluorescent Molecular Prodrug
12.1. Introduction
12.2. Reductive thiol-activated prodrug systems
12.3. Hydrogen peroxide-activated prodrug systems
12.4. Enzyme-activated prodrug systems
12.5. Acidic pH-activated prodrug systems
12.6. External light-activated prodrug systems
Chapter 13: Photosensitive Dyes for Photon-Triggered Cancer Therapy
13.1. Introduction
13.2. Photosensitive dyes for photodynamic therapy
13.2.1. Type II mechanism photosensitizers
13.2.2. Type I mechanism photosensitizers
13.2.3. Clinical photosensitive drugs
13.3. Photosensitive dyes for photothermal therapy
13.3.1. Porphyrins
13.3.2. Cyanines
13.3.3. Phthalocyanines
13.3.4. Diketopyrrolopyrrole dyes
13.3.5. Croconic acid dyes
13.3.6. Bodipys
13.4. Conclusion