The stratum corneum (SC) plays a crucial role in keeping an optimal skin barrier function, regulating the water-holding capacity. This experimental study shows that Dynamic Vapour Sorption (DVS) can be used to assess the hydration and dehydration characteristics of SC with intact or modified lipid structure. A future application of the DVS method could be the qualitative and quantitative evaluation of the damaging effect of chemical compounds, such as a single surfactant or a mixture of surfactants. We can directly test the finished product or the raw material before formulating.