Combinatorial Biotechnology: Methods for Synthesis of Peptide, Oligonucleotide and Oligosaccharide Libraries and Applications: Progress in Biochemistry and Biotechnology
Autor Preeti Srivastavaen Limba Engleză Paperback – iun 2026
- Provides comprehensive methods for the synthesis of peptide, oligonucleotide, and oligosaccharide libraries
- Covers both solid phase and solution phase synthesis techniques, including modern biological methods
- Examines encoding technologies and deconvolution methods essential for combinatorial biotechnology
- Offers valuable insights for researchers in protein engineering, molecular biology, and drug discovery
- Serves as a unique reference with no existing textbooks or reference books on this specialized subject
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Specificații
ISBN-13: 9780443275609
ISBN-10: 0443275602
Pagini: 325
Dimensiuni: 191 x 235 mm
Editura: ELSEVIER SCIENCE
Seria Progress in Biochemistry and Biotechnology
ISBN-10: 0443275602
Pagini: 325
Dimensiuni: 191 x 235 mm
Editura: ELSEVIER SCIENCE
Seria Progress in Biochemistry and Biotechnology
Cuprins
1. Solid Phase synthesis
2. Solid phase peptide synthesis
3. Solid phase Oligonucleotide synthesis
4. Solid phase oligosaccharide synthesis
5. Parallel peptide synthesis on solid phase Parallel synthesis: microplate method, Tea bag approach, Multi pin approach, SPOT synthesis, Light directed parallel synthesis, parallel synthesis using micro fluidic devices, peptide synthesis using peptide laser printer, cLIFT
6. Split only approach for peptide synthesis
7. Split and mix approach for peptide synthesis
8. Encoding Technologies Peptide encoding, Shaving, Oligonucleotide encoding, PNA encoding, Binary encoding, Mass encoding, optical encoding using fluorescent colloids, internal encoding, Physical encoding, electronic encoding, Graphical encoding.
9. Solution phase synthesis Dendrimers, Polymer supported reagents, scavenger resins, Fluorous reactants
10. Encoding in solution phase
11. Deconvolution methods Iterative deconvolution, Positional scanning, Orthogonal deconvolution, Subtractive deconvolution, Bogus coin deconvolution, Recursive deconvolution
12. Chemical Ligations Native Chemical ligation, Intein mediated ligation, Staudinger ligation
13. Biological methods: In vivo Display Libraries Filamentous phage display, lambda phage display, Bacterial cell surface display, Yeast cell surface display, Baculovirus capsid display, Mammalian cell surface display
14. Biological methods: In vitro display libraries Ribosomal display, mRNA display, CIS/CAD display, Plasmid display, In vitro compartmentalization
15. Genetic code reprogramming
2. Solid phase peptide synthesis
3. Solid phase Oligonucleotide synthesis
4. Solid phase oligosaccharide synthesis
5. Parallel peptide synthesis on solid phase Parallel synthesis: microplate method, Tea bag approach, Multi pin approach, SPOT synthesis, Light directed parallel synthesis, parallel synthesis using micro fluidic devices, peptide synthesis using peptide laser printer, cLIFT
6. Split only approach for peptide synthesis
7. Split and mix approach for peptide synthesis
8. Encoding Technologies Peptide encoding, Shaving, Oligonucleotide encoding, PNA encoding, Binary encoding, Mass encoding, optical encoding using fluorescent colloids, internal encoding, Physical encoding, electronic encoding, Graphical encoding.
9. Solution phase synthesis Dendrimers, Polymer supported reagents, scavenger resins, Fluorous reactants
10. Encoding in solution phase
11. Deconvolution methods Iterative deconvolution, Positional scanning, Orthogonal deconvolution, Subtractive deconvolution, Bogus coin deconvolution, Recursive deconvolution
12. Chemical Ligations Native Chemical ligation, Intein mediated ligation, Staudinger ligation
13. Biological methods: In vivo Display Libraries Filamentous phage display, lambda phage display, Bacterial cell surface display, Yeast cell surface display, Baculovirus capsid display, Mammalian cell surface display
14. Biological methods: In vitro display libraries Ribosomal display, mRNA display, CIS/CAD display, Plasmid display, In vitro compartmentalization
15. Genetic code reprogramming