With the implementation of No Child Left Behind (2004) and the requirements set IDEIA (2004), collaborative teaching has become a requirement for most special education and general education teachers. General education and special education teachers are asked to cooperate to successfully meet the needs of all students in their classroom and to ensure the execution of differentiated instruction in the inclusive classroom. This mixed methods research involves the observation, survey and interview of veteran general education and special education teachers. Specifically, the study was designed to verify participating teachers' perceptions of collaboration and how collaboration between general education and special education teachers is applied into the inclusive or the collaborative classroom. Interviews, observations and surveys were used to evaluate special education and general education teachers' perspectives of collaboration and its application in collaborative classrooms.