This book elucidates the concepts and innovative models around prospective developments with respect to chromatography. It describes in detail the various basic concepts of this field. Chromatography is the science and technique of using different laboratory methods to separate chemical mixtures. It is basically a method of purification. This textbook is a valuable compilation of topics, ranging from the basic to the most complete theories in the field of chromatography. Such selected concepts that redefine this subject have been presented in this text. Different approaches, evaluations and methodologies on chromatography have been included in this textbook. It will provide comprehensive knowledge to the students.