This book contains six chapters that detail chromatographic methods and research. Chapter One discusses how liquid chromatography-ultraviolet (LC-UV) bioanalytical methods can be considered an important tool for beta-lactams serum measurements to evaluate in a real time drug effectiveness in septic burn patient’s bedside. Chapter Two focuses on the preconcentration strategies coupled with chromatographic methods used for the detection of various types of organic compounds. Chapter Three details the bioanalytical methods, based on liquid chromatography, for the therapeutic drug monitoring of combined therapies of antibiotics in pediatric burns. Chapter Four presents liquid chromatography-tandem mass spectrometry. Chapter Five briefs about the determination of an antiviral drug, favipiravir (FVPR) in pure and pharmaceutical dosage forms and different biological matrix of samples, by employing various chromatographic techniques. Lastly, Chapter Six outlines key considerations for the analysis of the hormone α-MSH in brain tissue by the liquid chromatography tandem mass spectrometry (LC-MS/MS) method.