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Chlorophyll a Fluorescence

Editat de G C Papageorgiou, Govindjee
en Limba Engleză Paperback – 7 dec 2009

Structura acestui volum este concepută pentru a oferi o progresie riguroasă de la principiile biofizice fundamentale la metodologii complexe de diagnosticare a sănătății vegetale. Materialul este organizat metodologic pornind de la bazele istorice și fizica transferului de energie, continuând cu analiza detaliată a fotosistemelor I și II, și culminând cu aplicații de teledetecție. Remarcăm efortul editorial coordonat de G C Papageorgiou și Govindjee de a integra contribuțiile a 58 de specialiști într-un flux coerent ce explică de ce o fracțiune infimă de fotoni emiși sub formă de fluorescență reprezintă, de fapt, o semnătură digitală a stării metabolice a plantei. Descoperim aici o analiză sistematică a modului în care stresul de mediu — de la secetă la toxicitatea metalelor grele — modifică randamentul fotosintetic, oferind instrumente teoretice pentru interpretarea semnalelor PAM (Pulse-Amplitude-Modulation). Această abordare extinde cadrul propus de Practical Applications of Chlorophyll Fluorescence in Plant Biology de Jennifer R. DeEll cu date noi din sfera biophysics și a controlului fotoelectrochimic. Dacă lucrarea lui DeEll se concentra pe latura utilitară, volumul de față aprofundează mecanismele moleculare ale migrării excitației și capturii de fotoni în cianobacterii și alge roșii. Esențială pentru cercetarea avansată, lucrarea detaliază tranziția de la observația de laborator la monitorizarea globală prin satelit. Este un tratat de referință în Biochimie care transformă un fenomen optic aparent minor într-un indicator cantitativ precis pentru productivitatea agricolă și ecologia globală, fiind indispensabil în curricula de biologie moleculară și fiziologie vegetală.

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Specificații

ISBN-13: 9789048138821
ISBN-10: 9048138825
Pagini: 818
Ilustrații: XXIV, 818 p.
Dimensiuni: 196 x 262 x 38 mm
Greutate: 1.99 kg
Ediția:Softcover reprint of the original 1st ed. 2004
Editura: Springer
Locul publicării:Dordrecht, Netherlands

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De ce să citești această carte

Această lucrare reprezintă resursa definitivă pentru cercetătorii care doresc să stăpânească tehnica fluorescenței clorofilei ca instrument de diagnostic. Cititorul câștigă o înțelegere profundă a relației dintre transferul de electroni și adaptarea plantelor la stres. Este recomandată studenților la masterat și doctorat în biofizică și agronomie, oferind fundamentul teoretic necesar pentru utilizarea corectă a fluorometrelor moderne în evaluarea productivității ecosistemelor.


Descriere scurtă

Chlorophyll a Fluorescence: A Signature of Photosynthesis highlights chlorophyll (Chl) a fluorescence as a convenient, non-invasive, highly sensitive, rapid and quantitative probe of oxygenic photosynthesis. Thirty-one chapters, authored by 58 international experts, provide a solid foundation of the basic theory, as well as of the application of the rich information contained in the Chl a fluorescence signal as it relates to photosynthesis and plant productivity. Although the primary photochemical reactions of photosynthesis are highly efficient, a small fraction of absorbed photons escapes as Chl fluorescence, and this fraction varies with metabolic state, providing a basis for monitoring quantitatively various processes of photosynthesis. The book explains the mechanisms with which plants defend themselves against environmental stresses (excessive light, extreme temperatures, drought, hyper-osmolarity, heavy metals and UV). It also includes discussion on fluorescence imaging of leaves and cells and the remote sensing of Chl fluorescence from terrestrial, airborne, and satellite bases. The book is intended for use by graduate students, beginning researchers and advanced undergraduates in the areas of integrative plant biology, cellular and molecular biology, plant biology, biochemistry, biophysics, plant physiology, global ecology and agriculture.

Cuprins

Chlorophyll a Fluorescence: A Bit of Basics and History.- Fluorescence of Photosynthetic Pigments in Vitro and in Vivo.- Chlorophyll Fluorescence as a Probe of Photosynthetic Productivity.- Nuts and Bolts of Excitation Energy Migration and Energy Transfer.- Transfer and Trapping of Excitations in Plant Photosystems.- System Analysis and Photoelectrochemical Control of Chlorophyll Fluorescence in Terms of Trapping Models of Photosystem II: A Challenging View.- Photon Capture, Exciton Migration and Trapping and Fluorescence Emission in Cyanobacteria and Red Algae.- Photosystem II: Oxygen Evolution and Chlorophyll a Fluorescence Induced by Multiple Flashes.- Fluorescence of Photosystem I.- The Relationship between Photosynthetic Electron Transfer and its Regulation.- Pulse-Amplitude-Modulation (PAM) Fluorometry and Saturation Pulse Method: An Overview.- Analysis of the Chlorophyll a Fluorescence Transient.- Light Emission as a Probe of Charge Separation and Recombination in the Photosynthetic Apparatus: Relation of Prompt Fluorescence to Delayed Light Emission and Thermoluminescence.- Chlorophyll Fluorescence Imaging of Leaves and Fruits.- Using Chlorophyll a Fluorescence Imaging to Monitor Photosynthetic Performance.- Remote Sensing of Chlorophyll Fluorescence: Instrumentation and Analysis.- Probing the Mechanism of State Transitions in Oxygenic Photosynthesis by Chlorophyll Fluorescence Spectroscopy, Kinetics and Imaging.- Non-photochemical Energy Dissipation Determined by Chlorophyll Fluorescence Quenching: Characterization and Function.- Excess Light Stress: Multiple Dissipative Processes of Excess Excitation.- Using Mutants to Understand Light Stress Acclimation in Plants.- Excess Light Stress: Probing Excitation Dissipation Mechanisms through Global Analysis of Time-and Wavelength-Resolved Chlorophyll a Fluorescence.- Chlorophyll Fluorescence as a Tool to Monitor Plant Response to the Environment.- Plant Responses to Ultraviolet Radiation Stress.- Effects of Water Stress on the Photosynthetic Efficiency of Plants.- Chlorophyll a Fluorescence as a Probe of Heavy Metal Ion Toxicity in Plants.- Water and Solute Transport in Cyanobacteria as Probed by Chlorophyll Fluorescence.- Assembly of Light-Harvesting Complexes of Photosystem II and the Role of Chlorophyll b.- Light Adaptation and Senescence of the Photosynthetic Apparatus. Changes in Pigment Composition, Chlorophyll Fluorescence Parameters and Photosynthetic Activity.- From Leaves to Ecosystems: Using Chlorophyll Fluorescence to Assess Photosynthesis and Plant function in Ecological Studies.- Development and Application of Variable Chlorophyll Fluorescence Techniques in Marine Ecosystems.- Plant Productivity of Inland Waters.

Recenzii

From the reviews:
"...The subtitle of the book is very significant. Rather than merely describing in vivo Chla spectra, as the main title might imply, the book does concentrate on the "signature of photosynthesis" theme. The editors thereby create a highly practical textbook on the optical aspects of photosynthesis itself. " (Robert S. Knox, Photosynth. Res. 2005)
"...This excellently composed book is primarily intended for readers from advanced undergraduates to researchers in plant sciences, microbiology, biochemistry and biophysics. It can be widely recommended to a large readership and is much welcomed by the scientific community. For many years to come, this book will be the basic standard work on Chl fluorescence providing not only a broad overview on all aspects and application possibilities but – in addition - it will be the starting point for future research in this fascinating field of photosynthetic energy conversion and plant biology." (Claus Buschmann, J.Plant Physiol. 2005)
"...This book is one of the most comprehensive treatises on this subject. It is edited by .... two pioneers and leading experts in this field. The major importance of the present volume lies in providing the readers the wide spectrum of use of Chl a fluorescence technique and giving considerable guideline for its numerous applications. The book is, therefore, likely to satisfy both the professional and general readers." (B. Vani, S.R. Mishra and G.B. Kashpuri, Ind.J.Biochem. Biophys.2005)
"...This 19th volume of the series on Advances in Photosynthesis and Respiration (Series editor: Govindjee) is dedicated to L.N.M. Duysens whose simple, but elegant, experiments in the 1950s-1960s established the conceptual framework of the theoretical developments and several techniques related to the measurements of Chlorophyll (Chl) fluorescence and their diverse variants. The content of this magnificentvolume is replete with such instances. The book is a mine of information with lucid treatment of many aspects of fluorescence and photosynthesis that are significant in pure and applied fields. I have no doubt that the book will remain in vogue for many years to come and eventually end up as a classic reference decades later when the technologies overtake the contemporary levels so well discussed in this magnificent encyclopedic work. "(Swapan K. Bhattacharjee, Current Science. 2005)
"The editors have assembled a constellation of stars to illuminate the field of chlorophyll fluorescence. Any one even remotely interested in the subject will want to have a copy in their university or institute library … ." (Anthony W.D. Larkum, Phycologia, Vol. 45 (4), 2006)
“This encyclopaedic treatment of chlorophyll α fluorescence is Volume 19 in the Advances in Photosynthesis and Respiration series … . This is a large volume … amply illustrated and with several colour plates collected at the front … . Production is of a high quality … . this volume a very attractive purchase for the individual. … reference source for a multitude of information on this aspect of photosynthesis. It will be of huge value to students, postdocs and researchers embarking in this area.” (Malcolm J. Hawkesford, Biochemist e-volution, February, 2010)

Caracteristici

Deals with successful application of the use of chlorophyll fluorescence Detailed studies in the laboratory have been extended to measurement of total photosynthesis of cell, leaves, plants and plant ecosystems and also explains mechanisms for photoprotection of plants