Cell culture and tissue engineering are revolutionizing healthcare, biotechnology, and beyond. Cell culture serves as a cornerstone not only for tissue engineering but also for various fields, including biotechnology and fundamental biomedical research. Tissue engineering addresses critical medical challenges and advances healthcare by tackling issues such as organ shortages and enabling personalized medicine. This textbook is designed for both undergraduate and graduate students in the fields of bioengineering, biochemical engineering, biomedical engineering, biotechnology, and related disciplines. It also serves as a reference book for professionals in the field. Beginning with the basics of cell culture and cell engineering, this book lays a solid foundation for understanding the field of tissue engineering. Chapters on bioscaffolds, scaffold design, and bioreactors highlight the strategies used to create functional tissues. The book also covers tissue fabrication technologies including microfabrication, organoids, organs-on-a-chip, bioprinting, and decellularization. These techniques are presented with clarity, making complex concepts accessible to readers with varying levels of expertise. The last chapters focus on the practical and ethical aspects of tissue engineering. Finally, the book includes a detailed plan of eight hands-on lab training modules, making it an invaluable resource for students, instructors, and professionals in the field of tissue engineering.